Immuno_Cell Bio_Brochure

Immunology Reagents
Primary antibodies, secondary antibodies, conjugates,
control sera, enzymes and proteins
Cell Biology Products
Comprehensive cell culture media, reagents,
supplements and antibiotics
CRITICAL TOOLS FOR
LIFE SCIENCE RESEARCH
Immunology
& Cell Biology
MP BIOMEDICALS www.mpbio.com

 Immunology and Cell Biology
TABLE OF CONTENTS

Blood Fractionation: Maximize Separation Efficiency of Lymphocyte and FastGroTM, Fully Chemically Defined FBS Replacement
Polymorphonuclear Cells From Blood . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 for Cell Culture.............................................................. 18
Lymphocyte Separation forin-vitro Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Chemically-Defined Basal Media for Optimal Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . 19
Mononuclear Cell Isolation for Research Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Select Chemically-Defined Basal Cell Culture Media . . . . . . . . . . . . . . . . . . . . . . . . .19
Comprehensive Collection of Animal Sera for Immunoassays. . . . . . . . . . . . . . . . . . . . . . . 6
Cell Culture Antibiotics: Keep Your Cell Cultures Contamination Free. . . . . . . . . . . . . . . 20
Specialized Polyclonal Antibodies for Immunoassays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Select Antibiotics for Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
FITC-Conjugated Goat IgG Fraction to Achieve Detect and Eliminate Mycoplasma Contamination from Cell Cultures. . . . . . . . . . . . . . . . 21
the Highest Sensitivity and Specificity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 MP Biomedicals Mycoplasma Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Monoclonal Antibodies to Actin and Tubulin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Myco-Visible Mycoplasma LAMP Detection Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Anti-Human Collagen Monoclonal Antibody. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Obtain Superior Performance with High Purity Bovine Albumin Fraction V. . . . . . . . . . . . . 24
Other Popular Monoclonal Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Improve Sequence Coverage with Protease V8
for Protein Identification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
High Quality Secondary Antibodies from CappelTM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Superior Yeast Lysis Using Zymolyase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Antibodies to Rabbit Immunoglobulins. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Antibodies to Human Immunoglobulins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 Plant Cell Culture Media for Optimal Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Antibodies to Mouse Immunoglobulins. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Select Plant Cell/Tissue Media. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
High Quality and Consistent Animal Sera for Optimal Cell Culture. . . . . . . . . . . . . . . . . . 15 PlantCon™, Unique Design for Plant Cell and Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . 28
Fetal Bovine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Enzymes for Plant Cell Lysis and Protoplast Formation. . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Sera from Other Animals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
7X®: Detergent for Cell Culture, Instrument and Glassware Cleaning . . . . . . . . . . . . . . . 30
Chemically Defined FBS Replacement for Optimal Cell Culture . . . . . . . . . . . . . . . . . . . .17
Serum Replacement Promotes Long-Term Cell Culturing . . . . . . . . . . . . . . . . . . . . . 17

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 Immunology and Cell Biology
IMMUNOLOGY AND CELL BIOLOGY BLOOD FRACTIONATION:
RESEARCH SOLUTIONS Maximize Separation Efficiency of Lymphocyte
and Polymorphonuclear Cells From Blood
MP Biomedicals is a global leader in providing innovative immunological tools with the best quality,
flexibility and customization to accelerate your research. Antibodies have become critical tools for many Blood is composed of several cell types that need to be routinely isolated, such as monocytes,
areas of life science research, primarily for their use as molecular tags for specific labeling, separation, lymphocytes, and polymorphonuclear leukocytes. Isolation of mononuclear and polymorphonuclear
and detection. Our antibodies and other reagents have been recommended for use in most assays cells from blood serves as the starting point for a wide spectrum of immunology studies. One pain point
including western blot, immunoprecipitation, immunostaining, and flow cytometry. In addition to for many researchers is how to specifically isolate mononuclear and polymorphonuclear cells from blood
primary antibodies, we also offer a wide variety of secondary antibodies, control immunoglobulins, and with high yield and cell viability. We offer three products for the isolation of mononuclear and
control sera. polymorphonuclear cells from human peripheral blood, as well as bone marrow and umbilical cord blood.
Our comprehensive portfolio can meet all your needs in cell biology, spanning many areas of research Lymphocyte Isolation for Research Use
from cell structure, organization, function and metabolism, to life cycle. Our products include reagents, Lymphocyte Separation Medium (LSM™) is a legendary tool to separate lymphocytes from human
kits and solutions for cell separation, detachment, signaling, proliferation, growth, apoptosis, staining, peripheral blood, as well as bone marrow and umbilical cord blood. As proven by more than 2,200
and many more areas of current topics of cell biologists. Our time-tested and high quality products can scientific publications, it ensures:
provide the right tools for all your research needs. Maximum yield of monocytes Low endotoxin levels
>96% cell viability of lymphocytes Sterility
Easy and fast one-step centrifugation
LymphoSep™ Lymphocyte Separation Medium offers similar product features to our LSM, but it is
specifically designed for in-vitro isolation of lymphocytes from defibrinated, EDTA or heparinized treated
human blood.
Mononuclear and Polymorphonuclear Isolation in One Step
When it is necessary to separate both mononuclear and polymorphonuclear cells from blood, Mono-
Poly™ Resolving Medium (Mono-Poly™, M-PRM) may be used. Differential migration during centrifugation
allows for the resolution of both mononuclear and polymorphonuclear leukocytes into two distinct
bands that are relatively free of erythrocytes. This can be done in a one-step centrifugation process.
Description Size Cat. No.
Lymphocyte Separation Medium (LSM™) 5 x 100 mL 0850494
LymphoSep™ 500 mL 091692254
Mono-Poly™ Resolving Medium 100 mL 091698049

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 Immunology and Cell Biology
COMPREHENSIVE COLLECTION OF ANIMAL SERA SPECIALIZED POLYCLONAL ANTIBODIES
FOR IMMUNOASSAYS FOR IMMUNOASSAYS
Normal and whole sera are non-immune serum samples prepared from the blood Polyclonal antibodies represent a population of antibodies that are produced by different B cell clones
of healthy human, goat, mouse, rabbit, pig, or other species. They provide sufficient quantities of within the body by the immune response of an immunized animal. They are a collection of immunoglobulin
endogenous proteins to saturate and block nonspecific binding interactions for a wide range of molecules that react against a specific antigen, recognizing different epitopes within the antigen, and
immunological applications, including immunohistochemistry (IHC), ELISA, and Western blotting. We binding the antigen with varying affinities. These features of polyclonal antibodies prove to be
offer a wide range of high-quality, disease-free sera from a variety of species. advantageous over monoclonal antibodies in many biological assay applications, such as ELISA,
immunoprecipitation, and immunochemistry, due to their strong binding capacity to multiple epitopes.
Advantages and Features:
However, due to large variation during production and procedures, polyclonal antibodies are not always
High quality from healthy animals or donors offered with such high quality. With over 30 years of experience and expertise serving the antibody
Versatile for blocking or saturating nonspecific interactions research community, MP Biomedicals offers a large range of high quality specialized polyclonal antibodies
with various host and targets, ensuring:
Comprehensive collection from various species
Superior overall affinity to antigens Robust sensitivity of detection
Constant availability
High tolerance to changes, Trusted quality - validated by thousands
such as pH or buffer of scientific publications
Description Size Cat. No.
Description Cat. No.
Normal Goat Serum 50 mL 08642921
Anti-Hamster IgG from Goat Antibody 0856984
Normal Mouse Serum 10 mL 08642931 Anti-Human Red Blood Cells from Rabbit IgG Fraction 0855042
Normal Sheep Serum 50 mL 08642951 Anti-Sheep Red Blood Cells from Rabbit IgG Fraction 0855806
Normal Rat Serum 10 mL 08642941 Rabbit Antiserum to Sheep Red Blood Cells 0855800
Whole Horse Serum 2 mL 0855987 Rabbit Antiserum to Human Red Blood Cells 0855133
Guinea Pig Complement 0855852
Whole Swine Serum 2 mL 0855993
Rabbit IgG Fraction To ß-Galactosidase 08559761
Whole Mouse Serum 2 mL 0855989
Rabbit anti-GFP 08687361
Whole Bovine Serum 2 mL 0855980
Chicken anti-GFP 08687391
Whole Human Serum 2 mL 0855979
Goat IgG Fraction to Human Albumin 0855028
Whole Goat Serum 2 mL 0855984 Rabbit IgG Fraction to Human Albumin 0855029
Whole Hamster Serum 2 mL 0855986 Anti-Glucagon Polyclonal from Rabbit 0811184
Whole Chicken Serum 2 mL 0855982 Goat IgG Fraction to Hamster IgG 0855397

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 Immunology and Cell Biology
FITC-CONJUGATED GOAT IGG FRACTION TO MONOCLONAL ANTIBODIES
ACHIEVE THE HIGHEST SENSITIVITY AND TO ACTIN AND TUBULIN
SPECIFICITY
Antibodies to cytoskeletal proteins are widely used for protein loading controls or specific studies such
Due to its high absorptivity, excellent fluorescence quantum yield, and affordable pricing, fluorescein as apoptosis. MP Biomedicals antibodies to actin have demonstrated specificity directed towards all six
isothiocyanate (FITC) is among one of the most commonly used fluorescent dyes for flow cytometry, known vertebrate isoactins. Our anti-tubulin monoclonal antibodies enable researchers to visualize
immunohistochemistry, and fluorescent immunoassays. High quality FITC-conjugated goat IgG fractions microtubules in fixed cells and in fixed or frozen tissue sections from various species.
are designed to deliver optimal results, including:
Highest affinity to the target molecule Lowest non-specific binding Description Cat. No.
Outstanding fluorescence with No existence of Fc fragments 08691001
Monoclonal Antibody to Actin C4
optimal FITC conjugation 08691002
Monoclonal Anti-Alpha-Smooth Muscle Actin (Mouse Ascites Fluid),
08637931
Description Cat. No. Clone 1A4
Fluorescein-Conjugated Goat IgG Fraction to Human Complement C1Q 0855166 Mouse Anti-Actin Mab Clone B4 08691331
Fluorescein-Conjugated Goat IgG Fraction to Human Complement C3 0855167 Actin, Purified from Rabbit (as antigen positive control) 08771012
Fluorescein-Conjugated Goat IgG Fraction to Human Complement C4 0855168 ?-Tubulin Monoclonal Antibody 08691251
Fluorescein-Conjugated Goat IgG Fraction to Human Fibrinogen 0855169 ?-Tubulin Monoclonal Antibody 08691261
Fluorescein-Conjugated Goat IgG Fraction to Human IgG (Whole Molecule) 0855144
Fluorescein-Conjugated Goat IgG Fraction to Human IgM (5Fc µ) 0855153
Fluorescein-Conjugated Goat IgG Fraction to Human IgA (? Chain) 0855077
Other Fluorescein-Conjugated Antibody Products:
Description Cat. No.
Fluorescein-Conjugated Goat Affinity Purified Antibody to Mouse Immuno-
0855521
globulins (IgG, IgA, IgM)
Fluorescein-Conjugated Goat Affinity Purified F(ab’)2 Fragment to Mouse
0855526
Immunoglobulins (IgG, IgA, IgM)
Fluorescein-Conjugated Goat Affinity Purified F(ab’)2 Fragment to Rabbit IgG
0855665
(Whole Molecule)
Fluorescein-Conjugated Goat Affinity Purified Antibody to Human IgG
0855197
(Whole Molecule)
Fluorescein-Conjugated Goat Affinity Purified Antibody to Rat IgG (No Cross
0856407
to Human)
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 Immunology and Cell Biology
ANTI-HUMAN COLLAGEN OTHER POPULAR MONOCLONAL ANTIBODIES
MONOCLONAL ANTIBODY
MP Biomedicals offers highly validated recombinant monoclonal antibodies against biomarkers and other
Human collagen is the main structural protein of the extracellular space in various connective tissues. popular targets for biomedical research and bioprocessing.
These tissues make up the skin, bones, and tendons of the human body. It is the most abundant protein
High specificity and sensitivity to targets
in our bodies, making up about 30% of the whole-body protein content. Collagen consists of amino
High reproducibility
acids wound together to form triple-helices of elongated fibrils.
Minimal lot-to-lot variations
Due to these uninterrupted “Glycine-X-Y” triplet repeats in the collagen structure, it is often extremely
Bulk quantity
difficult to generate specific antibodies to collagens. However, we offers a series of anti-human collagen
monoclonal antibodies from mice to specifically and sensitively target a single type of human collagen
Description Cat. No.
without cross-reactivity with other collagen types. With quality validated by hundreds of journal citations,
Mouse, Anti-GFP, Monoclonal Antibody 08687371
our anti-human collagen monoclonal antibodies have been widely used for various applications, including
ELISA, immunoprecipitation, PAGE and western blotting. Mouse, Anti-? -Galactosidase, Purified Monoclonal Antibody 08633651
Specific binding to a single type of collagen Monoclonal Mouse Anti-Chondroitin-4-Sulfate Antibody 08636511
No cross-reactivity with other collagen types Monoclonal Mouse Anti-Chondroitin-6-Sulfate Antibody 08636521
Various immunochemical assay applications Mouse Anti-Synaptophysin IgG1 Monoclonal (Clone: SY38) 08697301
Validated by hundreds of journal citations Mouse Anti-Glial Fibrillary Acidic Protein (GFAP) Monoclonal 08691101
Anti-Human Hemoglobin Monoclonal Antibody from Mouse 08634801
Anti-Human IgG Monoclonal Antibody from Mouse 08634811
Description Cat. No.
Anti-Human Collagen Type I, Mouse Monoclonal Antibody Purified 08631702
Anti-Human Collagen Type II, Mouse (Clone II-4CII), Purified IgG 08631711
Anti-Human Collagen Type III, Mouse Monoclonal Antibody Purified 08631721
Anti-Human Collagen Type IV, Mouse Monoclonal Antibody, (Clone IV-4H12)
08631731
Purified IgG
Anti-Human Collagen Type V, Mouse Monoclonal Antibody Purified 08631741
Anti-Human Collagen Type VI, Mouse Monoclonal Antibody Purified 08631751
Anti-Human Collagen Type IX, Mouse Monoclonal Antibody Purified 08631761

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 Immunology and Cell Biology
HIGH QUALITY SECONDARY ANTIBODIES Antibodies to Human Immunoglobulins
Secondary antibodies are usually designed to bind to the primary antibody to amplify signals for Conjugate Host Fraction Size Cat. No.
detection, separation and quantification of the target antigen. To maximize signals, the secondary FITC Goat IgG 2 mL 0855077
antibody must have specificity to interact with the primary antibody species and isotype. In addition, a HRP Goat IgG 2 mL 0855215
secondary antibody usually has conjugation with a reporter molecule such as an enzyme or fluorophore. None Goat F(ab’)2 2 mL 0855049
MP Biomedicals offers a wide variety of secondary antibodies without conjugation or with enzyme/ None Goat Affinity Purified 2 mg 0855070
fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. FITC Rabbit IgG 2 mL 0855145
Enzyme (alkaline phosphatase (AP) or horseradish peroxidase (HRP)) conjugated antibodies are suitable FITC Goat F(ab’)2 2 mL 0855180
for EIA, ELISA, blot immunostaining and cell/tissue staining, while fluorochrome conjugated antibodies HRP Goat IgG 2 mL 0855220
are used for immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence- AP Goat Affinity Purified 1 mL 0859289
activated cell sorting. None Goat F(ab’)2 2 mL 0855053
High specificity for primary antibody species and isotypes None Goat Affinity Purified 1 mg 0855071
None Goat Affinity Purified F(ab’)2 2 mg 0856961
Multiple pre-conjugations for sensitive detection
HRP Goat F(ab’)2 2 mL 0855246
Various fragments
None Goat Affinity Purified F(ab’)2 2 mg 08670181
High purity FITC Goat IgG 2 mL 0855148
Validated by thousands of publications since 1960’s HRP Goat IgG 2 mL 0855224
None Goat IgG 5 mL 0855017
None Goat Affinity Purified F(ab’)2 1 mg 0856960
Antibodies to Rabbit Immunoglobulins FITC Goat IgG 2 mL 0855153
FITC Goat Affinity Purified 2 mg 0855199
Conjugate Host Fraction Size Cat. No.
None Goat Affinity Purified 2 mg 0855074
None Goat IgG 5 mL 0855622 FITC Goat IgG 2 mL 0855156
FITC Goat IgG 2 mL 0855676 FITC Goat F(ab’)2 2 mL 0855186
HRP Sheep IgG 2 mL 0855677 AP Goat Affinity Purified 1 mL 0859284
HRP Goat Affinity Purified 2 mL 0855689 FITC Goat IgG 2 mL 0855158
AP Goat Affinity Purified 1 mL 0859298 FITC Goat F(ab’)2 2 mL 0855188
None Goat Affinity Purified 2 mg 0855642 HRP Goat IgG 2 mL 0855233
FITC Goat F(ab’)2 10 mg 0855658 FITC Goat IgG 2 mL 0855159
None Goat Affinity Purified F(ab’)2 2 mg 08670391
HRP Goat Affinity Purified 2 mL 0855691

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 Immunology and Cell Biology
Antibodies to Mouse Immunoglobulins HIGH QUALITY AND CONSISTENT ANIMAL SERA FOR
OPTIMAL CELL CULTURE
Conjugate Host Fraction Size Cat. No.
None Goat Antiserum 2 mL 0855435 Animal sera, whether from bovine, human or other animal source, provide all essential nutrients such as
None Rabbit Antiserum 2 mL 0855436 proteins, attachment factors, growth factors, amino acids, trace elements, vitamins, lipids, and hormones
None Goat Affinity Purified 2 mg 0855479 for healthy cell culture growth. Additionally, animal serum plays an essential role in regulating cell
None Goat Affinity Purified 2 mg 08670281 osmotic pressure and membrane permeability, serving as a carrier for enzymes, micronutrients, lipids,
None Goat Affinity Purified F(ab’)2 2 mg 0855487 and trace elements into the cell. Therefore, animal serum has been widely used as a nutrient boost for
FITC Goat IgG 2 mL 0855493 most cell-culture applications in the life sciences.
FITC Sheep IgG 2 mL 0855495 To meet and exceed quality control standards for high performance in cell culture, all sera at MP
FITC Goat Affinity Purified 2 mg 08672281 Biomedicals are tested by independent third-party laboratories for the presence of endotoxins,
HRP Goat IgG 2 mL 0855550 mycoplasma, bacteria, fungi, and virus; for total protein concentration (including hemoglobin content);
HRP Goat Affinity Purified 2 mL 0855563 and for the growth of cells. A certificate of analysis for each lot is available upon request. Our animal sera
AP Sheep Affinity Purified 1 mL 0859293 ensure:
Biotin Goat Affinity Purified 2 mL 0855587
High performance for Minimized lot-to-lot variability
None Goat Affinity Purified 2 mg 0855482 broad cell types
Sterility
HRP Goat Affinity Purified 2 mL 0855566
Low endotoxin levels
AP Goat Affinity Purified 1 mL 0859297 Country of origin and traceability
Free of mycoplasma contamination
FITC Goat IgG 2 mL 0855499
FITC Goat Affinity Purified F(ab’)2 2 mg 0855526 Free of disease from animal sources
Rhod Goat Affinity Purified 2 mg 0855540
HRP Goat IgG 2 mL 0855556
HRP Goat Affinity Purified F(ab’)2 2 mL 0855576






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 Immunology and Cell Biology
Fetal Bovine Serum CHEMICALLY DEFINED FBS REPLACEMENT
Fetal bovine serum (FBS) is probably the most widely used serum-supplement for in vitro cell culture. FOR OPTIMAL CELL CULTURE
This is because FBS contains a complex array of protein components, excellent cell growth factors, low
endotoxin levels, and low hemoglobin concentration, all of which are required by cells to survive, grow, Serum Replacement Promotes Long-Term Cell Culturing
and divide. FBS from MP Biomedicals offers excellent value for basic cell culture, specialty research, and
Low-serum and serum-free media provide important advantages in animal cell culture, as the chemically
bioprocessing. With our quality assurance system from raw material to final product, we ensure reliable
controlled environment offers improved reproducibility and safety by removing lot-to-lot variation and
and consistent delivery of high quality FBS to our customers.
biorisk inherent to animal serum. TCMTM and TCHTM are designed to eliminate the use of serum in cell
culture, providing all essential components to promote cell growth and viability in most cell culture
Sera from Other Animals situations. Both serum replacements exhibit excellent results in long-term culturing of anchorage
dependent and suspension culture.
Although fetal bovine serum is the most commonly used serum for cell culture, many other animal sera
can be used based on cell origin, cross-reactivity, performance requirements, and cost, such as human Chemically defined nature
serum, newborn calf serum, horse serum, goat serum, rabbit serum, porcine serum, and chicken serum. Free of biological variability
We offer a wide spectrum of animal sera to meet your cell culture needs for both research and Free of growth factors or steroid hormones
bioprocessing.
Long-term culture with no chromosomal or morphological alterations
Description Cat. No. Versatile to any basic cell culture media
Low endotoxin levels
Fetal bovine serum (FBS), South-American origin 092910154B
Fetal bovine serum (FBS), South-American origin, heat 092910254 Low protein content to simplify downstream processing
and purification processes
Fetal Bovine Serum (South America). Tetracycline free 092917554
TCMTMis a fortified, multipurpose serum replacement for long-term culturing of many types of anchorage
Human Serum 092930149
dependent and suspension cultures with a variety of species, especially primary cell cultures. TCHTM is
Human Type AB Serum from Male Donors 092930949
particularly developed for long-term culturing of human cells.
Human Serum, Pooled 092931149
Rabbit Serum 092941149
Goat Serum 092939149 Description Size Cat. No.
Newborn Bovine Serum 092912149 TCMTM defined serum replacement, 50x concentrate 20 mL 092010026
Donor Horse Serum 092921149 TCHTM defined serum replacement, 50x concentrate 20 mL 092020026



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 Immunology and Cell Biology
FastGroTM, Fully Chemically Defined FBS Replacement CHEMICALLY-DEFINED BASAL MEDIA
for Cell Culture FOR OPTIMAL CELL CULTURE
Fetal bovine serum (FBS) is widely used as a serum-supplement for in vitro cell culture media, providing
Since the 1950’s, research scientists performing routine cell culture have been utilizing classical cell
an undefined mixture of nutrients for healthy cell culture growth, such as proteins, attachment factors,
culture media in countless applications. Minimizing the risk of adventitious agents or biological
growth factors, amino acids, trace elements, vitamins, lipids, and hormones. However, due to its
contamination can be critical to providing reliable, consistent, and high-quality results. MP Biomedicals
undefined nature and the variation of animals, FBS can lead to unexpected and undesired stimulations
supports researchers and scientists to enable them to reach their goals in their quest for scientific
of cells, not to mention biorisk of animal protein or pathogen contamination, such as bovine spongiform
excellence. A complete range of chemically-defined basal media are available to support optimal cell
encephalopathy (BSE).
growth, providing:
To avoid these concerns, MP Biomedicals is pleased to launch FastGro™, a fully chemically defined FBS
Chemically-defined essential components
replacement for cell culture use. This unique product allows culturing a wide range of cells in vitro
Lot-to-lot consistency
without the use of serum or any animal or human derived compound. All components in FastGro™ are
highly purified and identified chemical compounds, ensuring:
Animal-component free media
No proteins, hormones, or other growth factors
Chemically defined nature without lot-to-lot variations
No biological contaminations such as viruses, mycoplasma, or prions
No animal or human derived materials or compounds
Select Chemically-Defined Basal Cell Culture Media:
No interference with hormones or growth factors
Elimination of the risk of contaminants – viruses, mycoplasma, prions, etc. Description Cat. No.
Wide range of cell culture practices Basal Medium Eagle (BME) Vitamin Concentrate (100X) 091600449
Dulbecco’s Modification of Eagle’s Medium (1X Solution)
Storage in the refrigerator, and no need for thawing before use 091642954
With 4.5 g/L Dextrose, Without L-Glutamine and Inositol
Dulbecco’s Modification of Eagle’s Medium (1X Solution) Without
091642149
L- Glutamine, Leucine, Sodium Pyruvate
Description Size Cat. No. Dulbecco’s Modification of Eagle’s Medium (DMEM) (1X Solution) Without
091642754
L-Glutamine, Phenol Red
100 mL 092640049 Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank’s Salts,
FastGroTM, Fully Chemically Defined FBS Replacement for Cell Culture 091213254
0.35 g/L Sodium Bicarbonate Without L-Glutamine
500 mL 092640054
1X RPMI Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine 091646454
1X RPMI 1640 Without L-Glutamine and Phosphate,
091629754
With 0.85 g/L Sodium Bicarbonate
RPMI 1640 (1X Solution) Without L-Glutamine and L-Leucine 091629149
RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose 091646854
Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate 091050122

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 Immunology and Cell Biology
CELL CULTURE ANTIBIOTICS: KEEP YOUR DETECT AND ELIMINATE MYCOPLASMA
CELL CULTURES CONTAMINATION FREE CONTAMINATION FROM CELL CULTURES
In cell culture, contamination of the culture media with microbiological organisms like bacteria, yeast, One of the major issues in dealing with mammalian cell culture is infection due to mycoplasma. The
fungi, mycoplasma, and endotoxins can be extremely devastating, causing significant cell death and presence of mycoplasma can alter many cellular processes and cause malfunctioning of cells in proliferation,
even catastrophic loss of the entire culture. The detrimental impact of microbiological contamination protein synthesis, DNA transfection, etc. We offers a complete solution for mycoplasma contamination
needs to be aggressively treated. Currently, the availability of antibiotics provides an excellent opportunity in cell culture, from prevention, rapid detection (20 min) and sensitive detection (10 CFU/mL) to effective
to combat cell culture contamination with minimal impact on the mammalian cells being cultured. removal (fast and low cytotoxicity).
Judicious selection of the antibiotic for your specific cell lines and culture conditions allows for full
control of the media without altering cellular growth parameters. MP Biomedicals Mycoplasma Detection Kits
Whether you require an antibiotic active against gram-positive bacteria, gram-negative bacteria, yeast, Fast detection speed High sensitivity
or fungi, we provide a wide range of high-quality antibiotics to treat your cell culture contamination. Wide detection range of Premixed components
mycoplasma species for ease-of-use
Easy to use – Convenient addition to liquid culture medium
High potency – Keep your cell cultures contamination free
Myco-Sniff-Rapid Myco-Sniff Myco-Sniff-Valid
Broad spectrum – Effective against a wide range of microbial contaminants Mycoplasma Luciferase Mycoplasma PCR Mycoplasma PCR
Detection Kit Detection Kit Detection Kit
Select Antibiotics for Cell Culture: Method Luciferase assay PCR PCR
Time 20 min 3 hours 3 hours
Description Cat. No.
Sensitivity
091672346 High (50) Very High (20) Very High (10)
Amphotericin B, 250 µg/mL (Fungizone) (CFU/mL)
091672348
Specificity High Very High Very High
091676045
Gentamicin Sulfate Solution, 10 mg/mL
0916760J8
Once mycoplasma is detected in the cell culture medium, it is essential to eliminate it without losing any
091676245 cells or causing further contamination. Mycoplasma Removal Agent (MRA) is a reliable solution for
Gentamicin Reagent Solution, 50 mg/mL
0916762J8 mycoplasma removal and prevention.
091672048
G418 Sulfate, 50 mg/mL (Geneticin)
091672548 Prevent recontamination of the Compatible with most mammalian
culture at 0.1 µg/mL cell lines
Kanamycin Sulfate, 5 mg/mL 091672048
Sterility and low cytotoxicity
Penicillin-Streptomycin (10,000 IU/mL, 10 mg/mL) 091670249 Maintain cell viability
Penicillin-Streptomycin-Amphotericin B (100X) 091674049 Eliminate multiple mycoplasma Citation and recognition in
species within one week with the 550+ scientific publications
lowest dosage
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 Immunology and Cell Biology
Myco-Visible Mycoplasma LAMP Detection Kit
Myco-Visible Mycoplasma LAMP kit is intended for the detection of mycoplasma contaminations in cell
cultures. The kit achieves this by targeting and amplifying the 16S rRNA coding region of the Mycoplasma
genome at loci that are well conserved in all Mollicutes. The detection procedure can be completed within
45 mins and requires as little as 10 16S rRNA copies or 10 fg of Mycoplasma genomic DNA per reaction.
No cross-reactivity with other bacterial, fungal and mammalian DNA by Primer-BLAST. The test assay is
easy to perform with no thermal cycler required. Positive results can be visualized by color changes from
pink to yellow.
No Special Instrument Required: Direct detection with just a dry bath.
Rapid and Comprehensive: Detects Mycoplasma spp in 45 mins.
Sensitive: Requires as little as 10 16S rRNA copies or 10 fg of Mycoplasma genomic
DNA per reaction.
Easy to Use: Innovative colorimetric detection system - vivid color change.

1 week at RESULTS INTERPRETATION
0.5 µg/mL MRA
? 1. Test result shall be interpreted as positive if the reaction
solution turns yellow or orange.

2. Test result shall be interpreted as negative if the reaction
solution remains as pink.
Before Treatment After Treatment

Product Size Cat. No.
25 Tests 093050401
Myco-Sniff-Rapid™ Mycoplasma Luciferase Detection Kit
50 Tests 093050402
Myco-Sniff™ Mycoplasma PCR Detection Kit 48 Tests 093050201
Myco-Sniff-Valid™ Mycoplasma PCR Detection Kit 48 Tests 093050301
Mycoplasma Removal Agent 5 mL 093050044
Myco-Visible Mycoplasma LAMP Detection Kit 40 Preps 093050601

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 Immunology and Cell Biology
OBTAIN SUPERIOR PERFORMANCE WITH IMPROVE SEQUENCE COVERAGE WITH PROTEASE V8
HIGH PURITY BOVINE ALBUMIN FRACTION V FOR PROTEIN IDENTIFICATION
Bovine Albumin Fraction V (also known as Bovine Serum Albumin) is a serum albumin protein derived Successful peptide mapping requires selection of the right cleavage enzymes. Protease V8 (also known
from cows. It is commonly used in numerous biochemical applications including ELISA, as endoproteinase- Glu-C) complements tryptic digestion, specifically cleaving peptide bonds on the
immunohistochemistry, immunoblots, cell culture media, clinical chemistry reagents, and protein C-terminal side of glutamic and aspartic acids. Protease V8 from MP Biomedicals has been validated by
research. Our Bovine Albumin Fraction V is derived from healthy cows and ensures superior performance more than 200 scientific publications and provides:
for these applications.
Robust a