MP BIOMEDICALS

RNA Isolation &

Purification Kits

Perfect Solutions for your most Precious Samp les

Complete Kits including Specialized Lysing Matrix Tubes to make

Biological Sample Lysis Easy

High RNA yield, purity and integrity



20 YEARS OF EXPERTISE IN SAMPLE PREPARATION AND MOLECULAR BIOLOGY

MP BIOMEDICALS1 www.mpbio.com



 INTRODUCTION

Explore our Portfolio of RNA Extraction Kits to Find the Right Solution for your

Application

MP Biomedicals supplies a complete range of organic-based, spin column-based as well as magnetic beads-based

extraction kits for the isolation of high quality DNA-free RNA.

Efficient lysis of the starting material and simultaneous inactivation of endogenous RNases is achieved by mixing

the sample with the lysis buffer in a 2mL tube containing lysing matrix beads that is then processed in a FastPrep

homogenizer or equivalent bead beater.

After a centrifugation step to pellet the lysing matrix beads and cell debris, the RNA is purified with the reagents

supplied in the kit using a ready-to-use protocol.

Total RNA isolated with MP Biomedicals kits has a high purity and is ready-to-use for a broad panel of downstream

applications: Next-Gen sequencing, RT-PCR , cDNA-library, TaqMan® analysis and array technologies,

Northern Blot, RNA dot blots and in vitro translation.



The FastPrep System



A Complete Solution for Sample Preparation







Sample Holders Lysing Matrix Tubes DNA/RNA Extraction Kits

Any Size, Any Temperature 16 combinaison of beads to Ready-to-Use Protocols

grind any sample for DNA, RNA and Protein

Isolation from any Sample!

POWERFUL Thorough grinding and lysis of the most difficult samples in just a few seconds.

FLEXIBLE Easily interchangeable sample holders to process any sample size (2mL, 4.5mL, 15mL, 50mL

tubes, 250mL flasks and 96 well plates) at cryogenic or room temperature.

COMPLETE SOLUTION Large number of ready-to-use Lysing Matrix compositions and FastPrep Kits for

DNA, RNA and protein purification of any sample.

2



 RNA ISOLATION AND PURIFICATION KIT

SELECTION GUIDE

Sample Type Product Name Cat. No. Technology Prep No. Page

FastRNA Pro Green Kit 116045050 Organic Extraction 50 Preps 4

Human, animal and

SPINeasy RNA Kit for

plant tissues, cultured 116543050 Silica Spin Columns 50 Preps 5

Tissue

cells

Magbeads FastRNA Kit 116572096 Magnetic Beads 96 Preps 18

Formalin-fixed paraffin-

Magbeads FastRNA Kit 192

embedded tissues and 116573192 Magnetic Beads 18

for FFPE Preps

cells

Gram positive and FastRNA Pro Blue Kit 116025050 Organic extraction 50 Preps 7

gram negative bacteria,

bacterial spores, virus SPINeasy RNA Kit for

116541050 Silica spin columns 50 Preps 8

Bacteria

Bacteria Magbeads FastRNA Kit 116572096 Magnetic beads 96 Preps 18

Magbeads FastDNA/ 192

Virus 116571792 Magnetic beads 19

RNA Kit for virus Preps

FastRNA Pro Red Kit 116035050 Organic extraction 50 Preps 10

Yeast, fungi FastRNA SPIN Kit for

116030050 Silica spin columns 50 Preps 11

Yeast

Microorganisms and

other specimens FastRNA Pro Soil

116070050 Organic extraction 50 Preps 12

found in soil and other Direct Kit

and purification

environmental samples

with silica spin

(extraction from the FastRNA Pro Soil

116075050 filters

sample without pre- 50 Preps 13

Indirect Kit

wash step)

Any sample DNA/

SPINeasy DNA/RNA/

RNA and Protein from a 116544050 Silica spin columns 50 Preps 14

Protein All-In-One Kit

Single Sample

Agarose and

Silica and spin 200

polyacrylamide gels as RNaid SPIN Kit 111107200 15

filters Preps

well as any solution









3



 ANIMAL & PLANT TISSUES

FASTRNA PRO GREEN KIT

EFFICIENT: Lysis of human, animal, and plant tissues with

Lysing Matrix D tubes

CONSISTENT YIELDS: RNA isolation with the single-

reagent RNAPro solution

HIGH-QUALITY: RNA is ready-to-use for a variety of

downstream applications



REF

Organic Extraction 50 Preps 116045050



The FastRNA Pro Green Kit is optimized for purification of total RNA from human, animal and plant samples. Add

50-300 mg of tissue to a 2 ml tube containing Lysing Matrix D and the proprietary RNApro Solution. After one 40

second run in the FastPrep Instrument, total RNA is isolated via chloroform extraction and ethanol precipitation.

Tail Liver Brain Tail Liver Blood

0.24-9.5 kb

50- 2000bp

Kidney Ear RNA marker Kidney Brain DNA marker.

Figure 1 - Rat total RNA extracted with the FastRNA Pro Green Kit. Figure 2 - RT-PCR of actin genes from rat RNA extracted with the

Approximately 2% of the total RNA isolated from 100 mg frozen FastRNA Pro Green Kit. 20% of the RT-PCR reaction was loaded on

tissue was loaded on to a 1.2% denaturing agarose gel (1X MOPS). a 1.2% agarose gel (0.5X TAE).



4



 ANIMAL & PLANT TISSUES

SPINEASY RNA KIT FOR TISSUE

EFFICIENT : Lysis of any human, animal, plant tissue or cell

culture sample with Lysing Matrix A tubes

CONVENIENT : Silica spin column method for an easy

extraction process

HIGH-QUALITY : RNA is ready-to-use for a variety of

downstream applications

ENVIRONMENTALLY FRIENDLY: No toxic chemicals



REF

Silica Spin Columns 50 Preps 116543050

SPINeasy RNA Kit for Tissue is a silica-membrane spin-column kit that enables quick and convenient

purification of total RNA from various human/ animal tissues, plant tissues and tissue cultures.

The use of Lysing Matrix A tubes enables highly efficient lysis of tissue samples within seconds. Purified RNA is

of high quality and immediately ready for RT-PCR and other downstream applications.



Starting

Sample Extraction Results

Amount (mg)

Concentration A A

260/280 260/230

Rabbit Liver 21.4 626.46 2.08 2.19

Rabbit Spleen 14.0 310.53 2.04 2.26

Rabbit Kidney 22.3 237.88 2.06 2.21

Rabbit Lung 21.4 159.24 2.01 2.26

Rabbit Heart 22.5 54.56 2.01 2.82

Ginkgo Biloba Leaf 51.3 132.93 2.09 2.21

Prunus Davidiana

52.7 382.08 2.07 1.94

Leaf (wild peach)

Cherry Leaf 48.5 294.06 2.06 1.91

Begonia Leaf 52.0 368.66 2.06 1.94

Tomato Leaf 52.2 663.82 2.10 2.28

Peanut Leaf 70.2 898.92 2.10 2.31

Potato Leaf 52.4 491.20 2.14 2.30





5



 ANIMAL & PLANT TISSUES











Liver Spleen Kidney Lung Heart DNA Marker Ginkgo Prunus Cherry Begonia Tomato Peanut Potato

Biloba Davidian a Leaf Leaf Leaf Leaf Leaf

Leaf Leaf

Figure 1: RNA extracted from various samples using SPINeasy RNA Kit for Tissue, analyzed using agarose gel

electrophoresis.













DNA Liver Spleen Kidney Hear t Lung Negative Ginkgo Prunus Begonia Peanut Potato Negative

Marker Control Biloba Davidiana Leaf Leaf Leaf Control

( rabbit ?-actin) Leaf Leaf (Plant 18S)

Cherry Tomato

Leaf Leaf

Figure 2: RT-PCR amplification of RNA extracted from various samples using SPINeasy RNA Kit for Tissue.



Build your Molecular Biology Workflow

PCR Enzymes, Mastermixes and Kits



END-POINT PCR

REAL TIME PCR

REVERSE TRANSCRIPTION

DEOXYNUCLEOTIDES

6



 BACTERIA

FASTRNA PRO BLUE KIT

EFFICIENT: Lysis of any gram-positive and gram-negative

bacteria with Lysing Matrix B tubes

CONSISTENT YIELDS: RNA isolation with the single-reagent

RNAPro solution

HIGH-QUALITY: RNA is ready-to-use for a variety of

downstream applications



REF

Organic Extraction 50 Preps 116025050

The FastRNA Pro Blue Kit is optimized for purification of total RNA from both gram positive and gram negative

bacteria. Add a pellet of up to 1010cells in suspension to a 2 ml tube containing Lysing Matrix B and the proprietary

RNApro Solution. After one 40 second run in the FastPrep Instrument, total RNA is isolated via chloroform

extraction and ethanol precipitation.







Achieve Optimal Lysing Performance with FastPrep Lysing Matrix Tubes

Optimal cell disruption for any sample

Size of the beads and composition optimized according to the sample

No cross contamination with closed Lysing Matrix tubes

Available in 2ml, 4.5ml, 15ml , 50ml tubes or 96 well plates

Fit any high-speed bead-beating machines

7



 BACTERIA

SPINEASY RNA KIT FOR BACTERIA

EFFICIENT: Lysis of any gram-positive and gram-negative bacteria

with Lysing Matrix B tubes

CONVENIENT: Silica spin column method for an easy extraction

process

HIGH-QUALITY: RNA is ready-to-use for a variety of downstream

applications

ENVIRONMENTALLY FRIENDLY: No toxic chemicals

REF

Silica Spin Columns 50 Preps 116541050

SPINeasy RNA Kit for Bacteria is a silica-membrane spin-column kit that enables quick and convenient purification

of total RNA from gram-positive and gram-negative bacteria.A specially formulated RNASS solution that stabilizes

and protects RNA in bacteria samples is included in the kit.

The use of Lysing Matrix B tubes in combination with a FastPrep instrument enables highly efficient lysis of

bacterial samples within seconds.

Total RNA of high quality is purified within 40 minutes and is immediately available for RT-PCR and other

downstream applications.

Sample Starting Amount (mg) Extraction Results

Concentration A A

260/280 260/230

E. Coli Pellet from 3 mL overnight culture 763.59 2.10 2.26

S. Aureus Pellet from 3 mL overnight culture 349.47 2.13 2.04

E. Coli Negative

E. Coli S. Aureus

S. Aureus

Figure 1: Total RNA extracted from bacteria Figure 2: RT-PCR amplification of RNA extracted

samples using SPINeasy RNA Kit for Bacteria, from bacteria samples using SPINeasy RNA Kit for

analyzed using agarose gel electrophoresis. M: Bacteria. M: DNA marker; Lane 1: E. coli; Lane 2: S.

DNA marker; Lane 1: E. coli; Lane 2: S. aureus. aureus; Lane 3: Negative control.

8



 CASE STUDYCASE STUDY - BACTERIA

SARS-COV-2 RNA IN WASTEWATER

FastRNA Pro Blue Kit

Cat. No. 116025050









Shelesh Agrawal, Laura Orschler & Susanne Lackner.

Sci Rep 11, 5372 (2021).



A Proven-Method for the Isolation of pure RNA from Wastewater ready-to-use for monitoring the

SARS-CoV-2 RNA concentration in sewage

Long-term monitoring of SARS-CoV-2 RNA in wastewater of the Frankfurt metropolitan area in Southern Germany.

EFFICIENT MECHANICAL LYSIS OF VIRAL PARTICLES: with Lysing Matrix B tubes containing 0.1mm silica beads

included in the kit

HIGH YIELDS OF PURE RNA: with the RNApro reagent developed to protect RNA from degradation by RNases and

to isolate RNA via a phenol chloroform extraction method

SUCCESSFUL RT-qPCR ANALYSIS: extracted viral RNA was used as a template in a multiplex assay targeting the

N- , S- and ORF1ab viral genes.













Concentrations of SARS-CoV-2 RNA in the influent of the SARS-CoV-2 load as sum of the two WWTP influents

WasteWater Treatment Plant (WWTP) of Niederrad as determined as analyzed with RT-qPCR in comparison to the positive

by real-time qPCR tested COVID-19 cases in the city of Frankfurt am Main.







9



 YEAST & FUNGI

FASTRNA PRO RED KIT

EFFICIENT: Lysis of yeast and fungi with Lysing Matrix C tubes

CONSISTENT YIELDS: RNA isolation with the single-reagent

RNAPro solution

HIGH-QUALITY: RNA is ready-to-use for a variety of

downstream applications





REF

Organic Extraction 50 Preps 116035050



The FastRNA Pro Red Kit is optimized for purification of total RNA from yeast and fungi. Add a pellet of up to 1010

cells in suspension to a 2 ml tube containing Lysing Matrix C and the proprietary RNAPro Solution. After one 40

second run in the FastPrep Instrument, total RNA is isolated via chloroform extraction and ethanol precipitation.











Figure 1 - Yeast and fungal total RNA extracted with Figure 2 - RT-PCR of actin genes from yeast

the FastRNA Pro Red Kit. Approximately 2% of the total and fungal RNA extracted with the FastRNA

RNA isolated from 100 mg tissue or 1010 cells was loaded Pro Red Kit. 20% of the RT-PCR reaction was

onto a 1.2% denaturing agarose gel (1X MOPS). Lane 1: loaded on a 1.2% agarose gel (0.5X TAE).

S. pombe; Lane 2: S. cerevisiae; Lane 3: P. pastoris; Lane Lane 1: S. cerevisiae; Lane 2: P. pastoris;

4: C. albicans; Lane 5: common mushroom; Lane 6: small Lane 3: C. albicans; Lane 4: 50-2000bp DNA

common mushroom; Lane 7: 0.24-9.5kb RNA Ladder. marker.



10



 YEAST & FUNGI

FASTRNA SPIN KIT FOR YEAST

EFFICIENT: Lysis of yeast and fungi with Lysing Matrix Y tubes

RAPID AND REPRODUCIBLE: Purification of intact high quality

total RNA with the use of specialized spin columns

HIGH-QUALITY: RNA is ready-to-use for a variety of

downstream applications

ENVIRONMENTALLY FRIENDLY: No toxic chemicals

REF

Silica Spin Columns 50 Preps 116030050

The FastRNA SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast

strains and fungi in approximately 30 minutes using Lysing Matrix Y tubes for cell lysis and spin columns for the

purification process. High quality total RNA is eluted with RNase-free water. Both large and small RNA species

are recovered with this protocol.





Encapsulated Media



• NO WEIGHING • NO DUST • NO CLEANUP • NO SMELL



Hundreds of formulation for bacteria and yeast

Capsule format eliminates weighing, dust and cleanup

simply drop capsules in water and autoclave





11



 SOIL & ENVIRONMENTAL SAMPLES

The FastRNA Pro Soil-Direct and Indirect kits are designed to efficiently isolate total RNA from organic material

found in soil samples, soil supernatants or any other environmental samples.

The direct method consists of extracting nucleic acid from microorganisms and other biological specimens

directly from soil. The indirect method utilizes an initial separation of microorganisms and other biological

specimens from the soil followed by lysis of the organisms and RNA purification. FastRNA Pro Soil kits purify

RNA in a process that removes humic substances and other inhibitors and efficiently inactivates cellular RNases

during homogenization to prevent RNA degradation. Purified RNA is thus suitable for RT-PCR analysis and other

downstream applications.

FASTRNA PRO SOIL DIRECT KIT

RAPID & EFFICIENT: Thorough sample lysis in seconds of any soil

and environmental sample with Lysing Matrix E tubes

HIGH PURITY: 2 levels of purification to remove efficiently humic

acids and other inhibitors

RELIABLE: Cellular RNases are inactivated during homogenization to

prevent RNA degradation



REF

Organic Extraction 50 Preps 116070050

The FastRNA Pro Soil-Direct is designed to efficiently isolate total RNA from organic material found in soil

samples. Following processing with Lysing Matrix E in the FastPrep Instrument, samples are extracted with

chloroform and cleaned with RNAMATRIX and other proprietary reagents. Purified RNA is suitable for RT-PCR

analysis, Next-Gen Sequencing and other downstream applications.





Total RNA extracted from Soil Samples with the FastRNA®

Pro Soil-Direct Kit.Approximately 15% of the total RNA

isolated from 0.5 g of four different soil samples was loaded

on to a 0.8% agarose gel.





Soil #1 Soil #5 Soil #10 Soil #11

1 kb ladder



12



 SOIL & ENVIRONMENTAL SAMPLES





RT-PCR of Fungal Gene from Total RNA Isolated from

Soil Samples with the FastRNA® Pro Soil-Direct Kit.

Approximately 40% of the RT-PCR reaction was loaded on

to a 0.8% agarose gel.

150bp Soil #1 Soil #5 Soil #10 Soil #11

-2kb marker

FASTRNA PRO SOIL INDIRECT KIT

CLEAN: Initial separation of microorganisms and other

biological specimens from soil

RAPID & EFFICIENT: Thorough sample lysis in seconds of

any microorganism present in soil and environmental sample

with Lysing Matrix E tubes

CONSISTENT: Efficient removal of PCR inhibitors and

inactivation of cellular RNases during the homogenization

step



REF

Organic Extraction 50 Preps 116075050

The FastRNA Pro Soil Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil

samples or soil supernatants. Following processing with Lysing Matrix E in the FastPrep Instrument, samples

are extracted with chloroform and cleaned with RNAMATRIX and other proprietary reagents. Purified RNA is

suitable for RT-PCR analysis, Next-Gen Sequencing and other downstream applications.









Total RNA extracted from Soil Samples with the FastRNA® RT-PCR of Fungal Gene from Total RNA Isolated from

Pro Soil-Indirect Kit. Approximately 15% of the total RNA Soil Samples with the FastRNA® Pro Soil-Indirect Kit.

isolated from four different soil samples was loaded on to a Approximately 40% of the RT-PCR reaction was loaded

0.8% agarose gel. Lane 1: 1kb ladder, Lane 2: RNA from 5 g on to a 0.8% agarose gel. Lane 1: 150bp – 2kb marker,

Soil #1, Lane 3: RNA from 10 g Soil #2, Lane 4: RNA from 5 Lane 2: Soil #1, Lane 3: Soil #2, Lane 4: Soil #7, Lane 5:

g Soil #7, Lane 5: RNA from 5 g Soil #10. Soil #10.



13



 SIMULTANEOUS PURIFICATION OF RNA, DNA AND PROTEINS



SPINEASY DNA/RNA/PROTEINS ALL-IN-ONE KIT

RAPID: Isolate DNA/RNA/Protein from a single sample in one hour

EFFICIENT: Lysis of any resistant sample with Lysing Matrix A tubes

CONVENIENT: Easy-to-use silica spin column method for the DNA

and RNA extraction process

HIGH-QUALITY: DNA, RNA and proteins are suitable for a variety

of downstream applications

ENVIRONMENTALLY FRIENDLY: No toxic chemicals

REF

Silica Spin Columns 50 Preps 116544050

SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica-membrane spin columns

to isolate DNA, RNA and protein components from the same sample.

After an efficient cell lysis by bead beating with Lysing Matrix A tubes, DNA is adsorbed onto the first spin

column, while the flow-through is collected and used for RNA purification. The second spin column captures

RNA and the flow-through from this step is used for protein extraction.

DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted.

In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed and

resuspended.

Each molecular component is then immediately available for their respective downstream applications.



Starting

Sample Extraction Results

Amount (mg)

DNA RNA Protein

Yield A A Yield A A Yield

(µg/mg sample) 260/280 260/230 (µg/mg sample) 260/280 260/230 (µg/mg sample)

Kidney 17.7 1.28 1.83 2.42 1.30 2.04 2.23 14.12

Spleen 12.4 1.81 1.84 2.45 1.81 2.04 2.22 20.81

Liver 17.0 1.09 1.84 2.89 3.48 2.07 2.17 10.16



14



 Figure 1 (left): (A) DNA; (B) RNA; (C) protein extracted from each animal Figure 2 (right): (A) PCR amplification of DNA and (B) RT-PCR

tissue using SPINeasy DNA/RNA/Protein All-In-One Kit, analyzed using amplification of RNA extracted from various samples using SPINeasy

gel electrophoresis. M: DNA marker; Lane 1: Kidney; Lane 2: Spleen; Lane DNA/RNA/Protein All-In-One Kit. M: DNA marker; Lane 1: Kidney;

3: Liver. Lane 2: Spleen; Lane 3: Liver; Lane 4: Negative control.

RNA CLEAN UP

RNAID SPIN KIT

MAXIMUM RECOVERY: >90% recovery of RNA fragments of up to 10 kb

RAPID: 3 easy steps - bind, wash and elute

HIGH QUALITY: RNA is ready-to-use for any downstream application including reverse transcription

and Next-Gen Sequencing



REF

Silica and Spin Filters 200 Preps 111107200

The RNaid SPIN Kit contains all the reagents and materials necessary for the isolation and purification of RNA

from agarose and polyacrylamide gels as well as from any solution in 20 minutes.

This kit in combination with the FastDNA SPIN Kit for Soil (Cat N°: 116560200) is also used to co-extract

RNA and DNA from the same soil sample (protocol described in the article : Tournier E. et al, MethodsX. 2015 Mar

27;2:182-91).













15



 CASE STUDY

SIMULTANEOUS ISOLATION OF DNA AND RNA

FROM SOIL

Combined use of:

FastPrep-24 instrument

Cat. No. 116004500

FastDNA SPIN Kit for Soil

Cat. No. 116560200

RNaid SPIN Kit

Cat. No. 111107200



Tournier, E.; Amenc, L.; Pablo, A. L.; Legname, E.; Blanchart

, E.; Plassard, C.; Robin, A.; Bernard, L. MethodsX.

2015, 182.

A comparison of the composition of microbial communities based on co-extraction of both DNA and RNA provides insight

into the ecology of populations, provided that DNA and RNA are subjected to the same extraction bias. This study describes

the isolation of soil nucleic acids with simultaneous extraction and purification of DNA and RNA following a cascade scheme

involving the FastDNA SPIN Kit for Soil, the FastPrep instrument and the RNaid Kit and avoiding the use of harmful solvents

Reproducible: The coextraction protocol was optimized on a sandy clay loam soil. Yields of 26.7 µg of purified DNA

and 4.5 µg of purified RNA per gram of soil were obtained with very good repeatability for both DNA and RNA.

Reliable: The four tropical soils from Madagascar were extractable and gave various DNA and RNA recovery yields

sufficiently concentrated to be further analyzed by any other molecular technique.

Efficient:The protocol was efficient on different tropical soils, including Andosol, where their high contents of clays,

including poorly crystalline clays, and Fe and Al oxides typically make the nucleic acid extraction more difficult.















DNA (black) and RNA (light grey) extraction yields and their

respective 95% confidence intervals (alpha 0.05, 3 replicates) from

4 tropical soils, sampled in Madagascar and characterized by

different textures, mineralogies, metal and carbon contents.



16



 AUTOMATED NUCLEIC ACID PURIFICATION PLATFORM

MPURE-32 SYSTEM









FULLY AUTOMATED MAGNETIC BEAD-BASED

NUCLEIC ACID EXTRACTION SYSTEM





Fast and Consistent Results

Superior Purity and Yield

High-efficiency and stability





MPure-32™ aNAP System is a fully automatic platform for

magnetic reagent assays. It can process up to 32 samples

simultaneously within a short period of time (around 40

to 60 minutes). Still achieves Scientific and Diagnostic

requirement with safe operating procedure.





17



 MAGBEADS FASTRNA KIT

QUICK AND SIMPLE: RNA extraction from animal and plant tissues, cells in culture as well as bacteria

and yeast in one hour

FLEXIBLE: Manual workflow or easily adaptable to automated platforms

HIGH QUALITY : RNA is ready for all downstream applications including RT-PCR and Next-Gen

sequencing

ENVIRONMENTALLY FRIENDLY: No toxic chemicals



REF



Magnetic Beads 96 Preps 116572096

The Magbeads FastRNA Kit combines the speed and efficiency of silica-based technology with the convenient

handling of magnetic particles for purification of total RNA.

Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the

particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and

DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA

is eluted in RNase-free water.

MAGBEADS FASTRNA KIT FOR FFPE

SIMPLE AND RELIABLE: RNA extraction from tissue, cells and blood. A deparaffinization Solution is

included in the kit for an efficient paraffin removal.

FLEXIBLE: manual workflow or easily adaptable to automated platforms

HIGH QUALITY : RNA is ready for all downstream applications including RT-PCR and Next-Gen

sequencing

ENVIRONMENTALLY FRIENDLY: No toxic chemicals



REF



Magnetic Beads 192 Preps 116573192

The Magbeads FastRNA Kit for FFPE is based on the purification method of high binding magnetic particles. The

sample is lysed and digested. RNA is released into the lysate.

After addition of magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic

particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were

washed with washing buffer to remove the proteins and impurities, washed with ethanol to remove salts, and

finally the RNA was eluted with Elution Buffer.

18



 MAGBEADS FASTDNA/RNA KIT FOR VIRUS

SIMPLE AND RELIABLE: Total viral nucleic acid from cell-free/low-cell content biological samples such

as body fluids, serum, plasma as well as tissue homogenates and culture supernatant.

FLEXIBLE: manual workflow or easily adaptable to automated platforms

HIGH QUALITY : nucleic acids are suitable for a variety of downstream applications

ENVIRONMENTALLY FRIENDLY: No toxic chemicals



REF

Magnetic Beads 192 Preps 116571192



The Magbeads FastDNA/RNA Kit for Virus is based on the purification method of high binding magnetic particles.

The sample is lysed and digested with Protease. DNA/RNA is released into the lysate. After addition of magnetic

particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities

such as proteins will be removed without adsorption. The adsorbed particles are washed with washing solution

to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA is eluted with

Nuclease Free Water.





Need more information on our RNA Kits?

Visit our webpage







Would like to try one of our RNA Kits?

Request your FREE sample!







19



 MP BIOMEDICALS

AMERICAS: 800.854.0530 | custserv.na@mpbio.com www.mpbio.com

EUROPE: 00800.7777.9999 | custserv.eur@mpbio.com

APAC: +65 6775.0008 | custserv.ap@mpbio.com



20





MPure-96™

aNAP System

High-Throughput Automated

Nucleic Acid Extraction Platform

LS2023-06-09 MP BIOMEDICALS www.mpbio.com



 MPure-96™ aNAP System

Cat. No. 07EMC044D

MPure-96™ aNAP System is an automated nucleic

acid extraction platform designed for high-throughput

applications. Its specialized spin tips enable super

mixing efficiency of magnetic beads and larger

processing volume.

Features & Benefits



Easy-To-Use Highly Flexible Time Saving

Intuitive user interface and Customize your own protocols High-throughput

easy menu navigation. for any specific application or simultaneous processing

experimental requirements. of up to 96 samples.





Fully Automated Whirl Stirring

Mixing Technology

Flexibility in samples per run: 6 to 96. Simultaneous sample

processing and purification of nucleic acid. Enable automation Enable the sample processing of up to 1,600 ?L. Allow the

of your complicated manual steps. Independent temperature mixing of sample and reagents at the speed of up to 3,000 RPM.

control modules ensure the optimal assay performance. Effective prevention of cross contamination from aerosol.

Proven Perfomance

The performance of the MPure™ aNAP System instruments have been extensively evaluated

with MagBeads FastDNA® Kits. The following data show the high yield and purity of genomic

DNA (gDNA) extracted from various soil samples and feces samples.



Figure 1: gDNA extracted from Figure 2: gDNA extracted from

various soil samples using various soil samples using

MagBeads FastDNA® Kit for Soil. MagBeads FastDNA® Kit for Feces.



M : 1kb plus DNA ladder

Lane 1: 30 mg swine feces

M : 1kb plus DNA ladder Lane 2: 15 mg mouse feces

Lane 1: Organic soil 3 µL Lane 3: 30 mg human feces

Lane 2: Flowerbed soil 8 µL Lane 4: 150 mg chicken feces

Lane 3: Saline soil 8 µL Lane 5: 150 mg bovine feces

Lane 4: Desert soil 8 µL Lane 6: 150 mg elephant feces

Page - 2



 With the intuitive interface and flexible program,

MPure-96™ aNAP System will boost user’s

productivity by transforming routine operations into

a walk-away solution.



Specification

Model MPure-96™

Run time 25 - 60 min

Max. Throughput 96 samples/run

Flex Throughput with auto tubes 6, 12, 24, 48

Weight 95 kg

Dimensions (H x W x L) 700 mm (H) x 870 mm (W) x 575 mm (L)

Power Supply AC 220 - 240 V

Processing Volume 50 ?L - 1,600 ?L

Magnetic Rod >3,900 gauss

Spin Speed up to 3,000 RPM

Temperature Control 4 sets

Heating Block Yes (4pcs)

Heating RT~130 °C

UV & HEPA Available

Display 7-inch touchscreen

Consumables

6-tube format Autotube 16-base (for Autotube) 96-Deep-well Plate (Autoplate)

1536/box 300/box 100/box

117032149 117032150 117032151





96 Spin Tips Spin Tips

(Assembled Box) 20x1000 pcs

80/box 117096153

117096152

Page - 3



 MagBeads FastDNA® and FastRNA Kits

MP Biomedicals offers a range of DNA and RNA isolation kits named Magbeads kits designed

for various types of biological samples. MagBeads kits are available in two versions, one

including reagents in vials and one containing plates pre-filled with all the reagents and readyto-use

in combination with the MPure-96 platform.

Kits with reagents in vials

Cat. No. Product Name Pack size

116561050 MagBeads FastDNA® Kit for Soil 50 preps

116570400 MagBeads FastDNA® Kit for Feces 50 preps

116571192 MagBeads FastDNA/RNA® Kit for virus 192 preps

116572096 MagBeads FastRNA® Kit 96 preps

116573192 MagBeads FastRNA® Kit for FFPE 192 preps

MagBeads FastDNA® Kit

116575192 MagBeads FastDNA® Kit 192 preps Cat. No. 116575192

116574096 MagBeads FastDNA® Kit for Blood 96 preps 192 preps

116576096 MagBeads FastDNA® Kit for FFPE 96 preps

116577192 MagBeads Fast Circulating DNA Kit 192 preps

116578050 MagBeads FastRNA® Kit for Virus 50 preps

Kits with pre-filled plates ready-to-use with the MPure-96™

Cat. No. Product Name Pack size

117034100 MagBeads FastDNA® Kit for Soil 96 preps

117034200 MagBeads FastDNA® Kit for Feces 96 preps

117034300 MagBeads FastDNA/RNA® Kit for Virus 96 preps

117034400 MagBeads FastRNA® Kit 96 preps

MagBeads FastRNA® Kit for Virus

117034500 MagBeads FastRNA® Kit for FFPE 96 preps

Cat. No. 117036100

117034600 MagBeads FastDNA® Kit 96 preps 96 preps

117034700 MagBeads FastDNA® Kit for Blood 96 preps

117034800 MagBeads FastDNA® Kit for FFPE 96 preps

117034900 MagBeads Fast Circulating DNA Kit 96 preps

117036100 MagBeads FastRNA® Kit for Virus 96 preps



Need more information ?

Visit our MPure- 96™ webpage.



MP BIOMEDICALS

EUROPE: 00800.7777.9999 | custserv.eur@mpbio.com www.mpbio.com

AMERICAS: 800.854.0530 | custserv.na@mpbio.com

APAC: +65 6775.0008 | custserv.ap@mpbio.com

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