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RNA Isolation &
Purification Kits
Perfect Solutions for your most Precious Samp les
Complete Kits including Specialized Lysing Matrix Tubes to make
Biological Sample Lysis Easy
High RNA yield, purity and integrity
20 YEARS OF EXPERTISE IN SAMPLE PREPARATION AND MOLECULAR BIOLOGY
MP BIOMEDICALS1 www.mpbio.com
INTRODUCTION
Explore our Portfolio of RNA Extraction Kits to Find the Right Solution for your
Application
MP Biomedicals supplies a complete range of organic-based, spin column-based as well as magnetic beads-based
extraction kits for the isolation of high quality DNA-free RNA.
Efficient lysis of the starting material and simultaneous inactivation of endogenous RNases is achieved by mixing
the sample with the lysis buffer in a 2mL tube containing lysing matrix beads that is then processed in a FastPrep
homogenizer or equivalent bead beater.
After a centrifugation step to pellet the lysing matrix beads and cell debris, the RNA is purified with the reagents
supplied in the kit using a ready-to-use protocol.
Total RNA isolated with MP Biomedicals kits has a high purity and is ready-to-use for a broad panel of downstream
applications: Next-Gen sequencing, RT-PCR , cDNA-library, TaqMan® analysis and array technologies,
Northern Blot, RNA dot blots and in vitro translation.
The FastPrep System
A Complete Solution for Sample Preparation
Sample Holders Lysing Matrix Tubes DNA/RNA Extraction Kits
Any Size, Any Temperature 16 combinaison of beads to Ready-to-Use Protocols
grind any sample for DNA, RNA and Protein
Isolation from any Sample!
POWERFUL Thorough grinding and lysis of the most difficult samples in just a few seconds.
FLEXIBLE Easily interchangeable sample holders to process any sample size (2mL, 4.5mL, 15mL, 50mL
tubes, 250mL flasks and 96 well plates) at cryogenic or room temperature.
COMPLETE SOLUTION Large number of ready-to-use Lysing Matrix compositions and FastPrep Kits for
DNA, RNA and protein purification of any sample.
2
RNA ISOLATION AND PURIFICATION KIT
SELECTION GUIDE
Sample Type Product Name Cat. No. Technology Prep No. Page
FastRNA Pro Green Kit 116045050 Organic Extraction 50 Preps 4
Human, animal and
SPINeasy RNA Kit for
plant tissues, cultured 116543050 Silica Spin Columns 50 Preps 5
Tissue
cells
Magbeads FastRNA Kit 116572096 Magnetic Beads 96 Preps 18
Formalin-fixed paraffin-
Magbeads FastRNA Kit 192
embedded tissues and 116573192 Magnetic Beads 18
for FFPE Preps
cells
Gram positive and FastRNA Pro Blue Kit 116025050 Organic extraction 50 Preps 7
gram negative bacteria,
bacterial spores, virus SPINeasy RNA Kit for
116541050 Silica spin columns 50 Preps 8
Bacteria
Bacteria Magbeads FastRNA Kit 116572096 Magnetic beads 96 Preps 18
Magbeads FastDNA/ 192
Virus 116571792 Magnetic beads 19
RNA Kit for virus Preps
FastRNA Pro Red Kit 116035050 Organic extraction 50 Preps 10
Yeast, fungi FastRNA SPIN Kit for
116030050 Silica spin columns 50 Preps 11
Yeast
Microorganisms and
other specimens FastRNA Pro Soil
116070050 Organic extraction 50 Preps 12
found in soil and other Direct Kit
and purification
environmental samples
with silica spin
(extraction from the FastRNA Pro Soil
116075050 filters
sample without pre- 50 Preps 13
Indirect Kit
wash step)
Any sample DNA/
SPINeasy DNA/RNA/
RNA and Protein from a 116544050 Silica spin columns 50 Preps 14
Protein All-In-One Kit
Single Sample
Agarose and
Silica and spin 200
polyacrylamide gels as RNaid SPIN Kit 111107200 15
filters Preps
well as any solution
3
ANIMAL & PLANT TISSUES
FASTRNA PRO GREEN KIT
EFFICIENT: Lysis of human, animal, and plant tissues with
Lysing Matrix D tubes
CONSISTENT YIELDS: RNA isolation with the single-
reagent RNAPro solution
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
REF
Organic Extraction 50 Preps 116045050
The FastRNA Pro Green Kit is optimized for purification of total RNA from human, animal and plant samples. Add
50-300 mg of tissue to a 2 ml tube containing Lysing Matrix D and the proprietary RNApro Solution. After one 40
second run in the FastPrep Instrument, total RNA is isolated via chloroform extraction and ethanol precipitation.
Tail Liver Brain Tail Liver Blood
0.24-9.5 kb
50- 2000bp
Kidney Ear RNA marker Kidney Brain DNA marker.
Figure 1 - Rat total RNA extracted with the FastRNA Pro Green Kit. Figure 2 - RT-PCR of actin genes from rat RNA extracted with the
Approximately 2% of the total RNA isolated from 100 mg frozen FastRNA Pro Green Kit. 20% of the RT-PCR reaction was loaded on
tissue was loaded on to a 1.2% denaturing agarose gel (1X MOPS). a 1.2% agarose gel (0.5X TAE).
4
ANIMAL & PLANT TISSUES
SPINEASY RNA KIT FOR TISSUE
EFFICIENT : Lysis of any human, animal, plant tissue or cell
culture sample with Lysing Matrix A tubes
CONVENIENT : Silica spin column method for an easy
extraction process
HIGH-QUALITY : RNA is ready-to-use for a variety of
downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116543050
SPINeasy RNA Kit for Tissue is a silica-membrane spin-column kit that enables quick and convenient
purification of total RNA from various human/ animal tissues, plant tissues and tissue cultures.
The use of Lysing Matrix A tubes enables highly efficient lysis of tissue samples within seconds. Purified RNA is
of high quality and immediately ready for RT-PCR and other downstream applications.
Starting
Sample Extraction Results
Amount (mg)
Concentration A A
260/280 260/230
Rabbit Liver 21.4 626.46 2.08 2.19
Rabbit Spleen 14.0 310.53 2.04 2.26
Rabbit Kidney 22.3 237.88 2.06 2.21
Rabbit Lung 21.4 159.24 2.01 2.26
Rabbit Heart 22.5 54.56 2.01 2.82
Ginkgo Biloba Leaf 51.3 132.93 2.09 2.21
Prunus Davidiana
52.7 382.08 2.07 1.94
Leaf (wild peach)
Cherry Leaf 48.5 294.06 2.06 1.91
Begonia Leaf 52.0 368.66 2.06 1.94
Tomato Leaf 52.2 663.82 2.10 2.28
Peanut Leaf 70.2 898.92 2.10 2.31
Potato Leaf 52.4 491.20 2.14 2.30
5
ANIMAL & PLANT TISSUES
Liver Spleen Kidney Lung Heart DNA Marker Ginkgo Prunus Cherry Begonia Tomato Peanut Potato
Biloba Davidian a Leaf Leaf Leaf Leaf Leaf
Leaf Leaf
Figure 1: RNA extracted from various samples using SPINeasy RNA Kit for Tissue, analyzed using agarose gel
electrophoresis.
DNA Liver Spleen Kidney Hear t Lung Negative Ginkgo Prunus Begonia Peanut Potato Negative
Marker Control Biloba Davidiana Leaf Leaf Leaf Control
( rabbit ?-actin) Leaf Leaf (Plant 18S)
Cherry Tomato
Leaf Leaf
Figure 2: RT-PCR amplification of RNA extracted from various samples using SPINeasy RNA Kit for Tissue.
Build your Molecular Biology Workflow
PCR Enzymes, Mastermixes and Kits
END-POINT PCR
REAL TIME PCR
REVERSE TRANSCRIPTION
DEOXYNUCLEOTIDES
6
BACTERIA
FASTRNA PRO BLUE KIT
EFFICIENT: Lysis of any gram-positive and gram-negative
bacteria with Lysing Matrix B tubes
CONSISTENT YIELDS: RNA isolation with the single-reagent
RNAPro solution
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
REF
Organic Extraction 50 Preps 116025050
The FastRNA Pro Blue Kit is optimized for purification of total RNA from both gram positive and gram negative
bacteria. Add a pellet of up to 1010cells in suspension to a 2 ml tube containing Lysing Matrix B and the proprietary
RNApro Solution. After one 40 second run in the FastPrep Instrument, total RNA is isolated via chloroform
extraction and ethanol precipitation.
Achieve Optimal Lysing Performance with FastPrep Lysing Matrix Tubes
Optimal cell disruption for any sample
Size of the beads and composition optimized according to the sample
No cross contamination with closed Lysing Matrix tubes
Available in 2ml, 4.5ml, 15ml , 50ml tubes or 96 well plates
Fit any high-speed bead-beating machines
7
BACTERIA
SPINEASY RNA KIT FOR BACTERIA
EFFICIENT: Lysis of any gram-positive and gram-negative bacteria
with Lysing Matrix B tubes
CONVENIENT: Silica spin column method for an easy extraction
process
HIGH-QUALITY: RNA is ready-to-use for a variety of downstream
applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116541050
SPINeasy RNA Kit for Bacteria is a silica-membrane spin-column kit that enables quick and convenient purification
of total RNA from gram-positive and gram-negative bacteria.A specially formulated RNASS solution that stabilizes
and protects RNA in bacteria samples is included in the kit.
The use of Lysing Matrix B tubes in combination with a FastPrep instrument enables highly efficient lysis of
bacterial samples within seconds.
Total RNA of high quality is purified within 40 minutes and is immediately available for RT-PCR and other
downstream applications.
Sample Starting Amount (mg) Extraction Results
Concentration A A
260/280 260/230
E. Coli Pellet from 3 mL overnight culture 763.59 2.10 2.26
S. Aureus Pellet from 3 mL overnight culture 349.47 2.13 2.04
E. Coli Negative
E. Coli S. Aureus
S. Aureus
Figure 1: Total RNA extracted from bacteria Figure 2: RT-PCR amplification of RNA extracted
samples using SPINeasy RNA Kit for Bacteria, from bacteria samples using SPINeasy RNA Kit for
analyzed using agarose gel electrophoresis. M: Bacteria. M: DNA marker; Lane 1: E. coli; Lane 2: S.
DNA marker; Lane 1: E. coli; Lane 2: S. aureus. aureus; Lane 3: Negative control.
8
CASE STUDYCASE STUDY - BACTERIA
SARS-COV-2 RNA IN WASTEWATER
FastRNA Pro Blue Kit
Cat. No. 116025050
Shelesh Agrawal, Laura Orschler & Susanne Lackner.
Sci Rep 11, 5372 (2021).
A Proven-Method for the Isolation of pure RNA from Wastewater ready-to-use for monitoring the
SARS-CoV-2 RNA concentration in sewage
Long-term monitoring of SARS-CoV-2 RNA in wastewater of the Frankfurt metropolitan area in Southern Germany.
EFFICIENT MECHANICAL LYSIS OF VIRAL PARTICLES: with Lysing Matrix B tubes containing 0.1mm silica beads
included in the kit
HIGH YIELDS OF PURE RNA: with the RNApro reagent developed to protect RNA from degradation by RNases and
to isolate RNA via a phenol chloroform extraction method
SUCCESSFUL RT-qPCR ANALYSIS: extracted viral RNA was used as a template in a multiplex assay targeting the
N- , S- and ORF1ab viral genes.
Concentrations of SARS-CoV-2 RNA in the influent of the SARS-CoV-2 load as sum of the two WWTP influents
WasteWater Treatment Plant (WWTP) of Niederrad as determined as analyzed with RT-qPCR in comparison to the positive
by real-time qPCR tested COVID-19 cases in the city of Frankfurt am Main.
9
YEAST & FUNGI
FASTRNA PRO RED KIT
EFFICIENT: Lysis of yeast and fungi with Lysing Matrix C tubes
CONSISTENT YIELDS: RNA isolation with the single-reagent
RNAPro solution
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
REF
Organic Extraction 50 Preps 116035050
The FastRNA Pro Red Kit is optimized for purification of total RNA from yeast and fungi. Add a pellet of up to 1010
cells in suspension to a 2 ml tube containing Lysing Matrix C and the proprietary RNAPro Solution. After one 40
second run in the FastPrep Instrument, total RNA is isolated via chloroform extraction and ethanol precipitation.
Figure 1 - Yeast and fungal total RNA extracted with Figure 2 - RT-PCR of actin genes from yeast
the FastRNA Pro Red Kit. Approximately 2% of the total and fungal RNA extracted with the FastRNA
RNA isolated from 100 mg tissue or 1010 cells was loaded Pro Red Kit. 20% of the RT-PCR reaction was
onto a 1.2% denaturing agarose gel (1X MOPS). Lane 1: loaded on a 1.2% agarose gel (0.5X TAE).
S. pombe; Lane 2: S. cerevisiae; Lane 3: P. pastoris; Lane Lane 1: S. cerevisiae; Lane 2: P. pastoris;
4: C. albicans; Lane 5: common mushroom; Lane 6: small Lane 3: C. albicans; Lane 4: 50-2000bp DNA
common mushroom; Lane 7: 0.24-9.5kb RNA Ladder. marker.
10
YEAST & FUNGI
FASTRNA SPIN KIT FOR YEAST
EFFICIENT: Lysis of yeast and fungi with Lysing Matrix Y tubes
RAPID AND REPRODUCIBLE: Purification of intact high quality
total RNA with the use of specialized spin columns
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116030050
The FastRNA SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast
strains and fungi in approximately 30 minutes using Lysing Matrix Y tubes for cell lysis and spin columns for the
purification process. High quality total RNA is eluted with RNase-free water. Both large and small RNA species
are recovered with this protocol.
Encapsulated Media
• NO WEIGHING • NO DUST • NO CLEANUP • NO SMELL
Hundreds of formulation for bacteria and yeast
Capsule format eliminates weighing, dust and cleanup
simply drop capsules in water and autoclave
11
SOIL & ENVIRONMENTAL SAMPLES
The FastRNA Pro Soil-Direct and Indirect kits are designed to efficiently isolate total RNA from organic material
found in soil samples, soil supernatants or any other environmental samples.
The direct method consists of extracting nucleic acid from microorganisms and other biological specimens
directly from soil. The indirect method utilizes an initial separation of microorganisms and other biological
specimens from the soil followed by lysis of the organisms and RNA purification. FastRNA Pro Soil kits purify
RNA in a process that removes humic substances and other inhibitors and efficiently inactivates cellular RNases
during homogenization to prevent RNA degradation. Purified RNA is thus suitable for RT-PCR analysis and other
downstream applications.
FASTRNA PRO SOIL DIRECT KIT
RAPID & EFFICIENT: Thorough sample lysis in seconds of any soil
and environmental sample with Lysing Matrix E tubes
HIGH PURITY: 2 levels of purification to remove efficiently humic
acids and other inhibitors
RELIABLE: Cellular RNases are inactivated during homogenization to
prevent RNA degradation
REF
Organic Extraction 50 Preps 116070050
The FastRNA Pro Soil-Direct is designed to efficiently isolate total RNA from organic material found in soil
samples. Following processing with Lysing Matrix E in the FastPrep Instrument, samples are extracted with
chloroform and cleaned with RNAMATRIX and other proprietary reagents. Purified RNA is suitable for RT-PCR
analysis, Next-Gen Sequencing and other downstream applications.
Total RNA extracted from Soil Samples with the FastRNA®
Pro Soil-Direct Kit.Approximately 15% of the total RNA
isolated from 0.5 g of four different soil samples was loaded
on to a 0.8% agarose gel.
Soil #1 Soil #5 Soil #10 Soil #11
1 kb ladder
12
SOIL & ENVIRONMENTAL SAMPLES
RT-PCR of Fungal Gene from Total RNA Isolated from
Soil Samples with the FastRNA® Pro Soil-Direct Kit.
Approximately 40% of the RT-PCR reaction was loaded on
to a 0.8% agarose gel.
150bp Soil #1 Soil #5 Soil #10 Soil #11
-2kb marker
FASTRNA PRO SOIL INDIRECT KIT
CLEAN: Initial separation of microorganisms and other
biological specimens from soil
RAPID & EFFICIENT: Thorough sample lysis in seconds of
any microorganism present in soil and environmental sample
with Lysing Matrix E tubes
CONSISTENT: Efficient removal of PCR inhibitors and
inactivation of cellular RNases during the homogenization
step
REF
Organic Extraction 50 Preps 116075050
The FastRNA Pro Soil Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil
samples or soil supernatants. Following processing with Lysing Matrix E in the FastPrep Instrument, samples
are extracted with chloroform and cleaned with RNAMATRIX and other proprietary reagents. Purified RNA is
suitable for RT-PCR analysis, Next-Gen Sequencing and other downstream applications.
Total RNA extracted from Soil Samples with the FastRNA® RT-PCR of Fungal Gene from Total RNA Isolated from
Pro Soil-Indirect Kit. Approximately 15% of the total RNA Soil Samples with the FastRNA® Pro Soil-Indirect Kit.
isolated from four different soil samples was loaded on to a Approximately 40% of the RT-PCR reaction was loaded
0.8% agarose gel. Lane 1: 1kb ladder, Lane 2: RNA from 5 g on to a 0.8% agarose gel. Lane 1: 150bp – 2kb marker,
Soil #1, Lane 3: RNA from 10 g Soil #2, Lane 4: RNA from 5 Lane 2: Soil #1, Lane 3: Soil #2, Lane 4: Soil #7, Lane 5:
g Soil #7, Lane 5: RNA from 5 g Soil #10. Soil #10.
13
SIMULTANEOUS PURIFICATION OF RNA, DNA AND PROTEINS
SPINEASY DNA/RNA/PROTEINS ALL-IN-ONE KIT
RAPID: Isolate DNA/RNA/Protein from a single sample in one hour
EFFICIENT: Lysis of any resistant sample with Lysing Matrix A tubes
CONVENIENT: Easy-to-use silica spin column method for the DNA
and RNA extraction process
HIGH-QUALITY: DNA, RNA and proteins are suitable for a variety
of downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116544050
SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica-membrane spin columns
to isolate DNA, RNA and protein components from the same sample.
After an efficient cell lysis by bead beating with Lysing Matrix A tubes, DNA is adsorbed onto the first spin
column, while the flow-through is collected and used for RNA purification. The second spin column captures
RNA and the flow-through from this step is used for protein extraction.
DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted.
In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed and
resuspended.
Each molecular component is then immediately available for their respective downstream applications.
Starting
Sample Extraction Results
Amount (mg)
DNA RNA Protein
Yield A A Yield A A Yield
(µg/mg sample) 260/280 260/230 (µg/mg sample) 260/280 260/230 (µg/mg sample)
Kidney 17.7 1.28 1.83 2.42 1.30 2.04 2.23 14.12
Spleen 12.4 1.81 1.84 2.45 1.81 2.04 2.22 20.81
Liver 17.0 1.09 1.84 2.89 3.48 2.07 2.17 10.16
14
Figure 1 (left): (A) DNA; (B) RNA; (C) protein extracted from each animal Figure 2 (right): (A) PCR amplification of DNA and (B) RT-PCR
tissue using SPINeasy DNA/RNA/Protein All-In-One Kit, analyzed using amplification of RNA extracted from various samples using SPINeasy
gel electrophoresis. M: DNA marker; Lane 1: Kidney; Lane 2: Spleen; Lane DNA/RNA/Protein All-In-One Kit. M: DNA marker; Lane 1: Kidney;
3: Liver. Lane 2: Spleen; Lane 3: Liver; Lane 4: Negative control.
RNA CLEAN UP
RNAID SPIN KIT
MAXIMUM RECOVERY: >90% recovery of RNA fragments of up to 10 kb
RAPID: 3 easy steps - bind, wash and elute
HIGH QUALITY: RNA is ready-to-use for any downstream application including reverse transcription
and Next-Gen Sequencing
REF
Silica and Spin Filters 200 Preps 111107200
The RNaid SPIN Kit contains all the reagents and materials necessary for the isolation and purification of RNA
from agarose and polyacrylamide gels as well as from any solution in 20 minutes.
This kit in combination with the FastDNA SPIN Kit for Soil (Cat N°: 116560200) is also used to co-extract
RNA and DNA from the same soil sample (protocol described in the article : Tournier E. et al, MethodsX. 2015 Mar
27;2:182-91).
15
CASE STUDY
SIMULTANEOUS ISOLATION OF DNA AND RNA
FROM SOIL
Combined use of:
FastPrep-24 instrument
Cat. No. 116004500
FastDNA SPIN Kit for Soil
Cat. No. 116560200
RNaid SPIN Kit
Cat. No. 111107200
Tournier, E.; Amenc, L.; Pablo, A. L.; Legname, E.; Blanchart
, E.; Plassard, C.; Robin, A.; Bernard, L. MethodsX.
2015, 182.
A comparison of the composition of microbial communities based on co-extraction of both DNA and RNA provides insight
into the ecology of populations, provided that DNA and RNA are subjected to the same extraction bias. This study describes
the isolation of soil nucleic acids with simultaneous extraction and purification of DNA and RNA following a cascade scheme
involving the FastDNA SPIN Kit for Soil, the FastPrep instrument and the RNaid Kit and avoiding the use of harmful solvents
Reproducible: The coextraction protocol was optimized on a sandy clay loam soil. Yields of 26.7 µg of purified DNA
and 4.5 µg of purified RNA per gram of soil were obtained with very good repeatability for both DNA and RNA.
Reliable: The four tropical soils from Madagascar were extractable and gave various DNA and RNA recovery yields
sufficiently concentrated to be further analyzed by any other molecular technique.
Efficient:The protocol was efficient on different tropical soils, including Andosol, where their high contents of clays,
including poorly crystalline clays, and Fe and Al oxides typically make the nucleic acid extraction more difficult.
DNA (black) and RNA (light grey) extraction yields and their
respective 95% confidence intervals (alpha 0.05, 3 replicates) from
4 tropical soils, sampled in Madagascar and characterized by
different textures, mineralogies, metal and carbon contents.
16
AUTOMATED NUCLEIC ACID PURIFICATION PLATFORM
MPURE-32 SYSTEM
FULLY AUTOMATED MAGNETIC BEAD-BASED
NUCLEIC ACID EXTRACTION SYSTEM
Fast and Consistent Results
Superior Purity and Yield
High-efficiency and stability
MPure-32™ aNAP System is a fully automatic platform for
magnetic reagent assays. It can process up to 32 samples
simultaneously within a short period of time (around 40
to 60 minutes). Still achieves Scientific and Diagnostic
requirement with safe operating procedure.
17
MAGBEADS FASTRNA KIT
QUICK AND SIMPLE: RNA extraction from animal and plant tissues, cells in culture as well as bacteria
and yeast in one hour
FLEXIBLE: Manual workflow or easily adaptable to automated platforms
HIGH QUALITY : RNA is ready for all downstream applications including RT-PCR and Next-Gen
sequencing
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Magnetic Beads 96 Preps 116572096
The Magbeads FastRNA Kit combines the speed and efficiency of silica-based technology with the convenient
handling of magnetic particles for purification of total RNA.
Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the
particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and
DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA
is eluted in RNase-free water.
MAGBEADS FASTRNA KIT FOR FFPE
SIMPLE AND RELIABLE: RNA extraction from tissue, cells and blood. A deparaffinization Solution is
included in the kit for an efficient paraffin removal.
FLEXIBLE: manual workflow or easily adaptable to automated platforms
HIGH QUALITY : RNA is ready for all downstream applications including RT-PCR and Next-Gen
sequencing
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Magnetic Beads 192 Preps 116573192
The Magbeads FastRNA Kit for FFPE is based on the purification method of high binding magnetic particles. The
sample is lysed and digested. RNA is released into the lysate.
After addition of magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic
particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were
washed with washing buffer to remove the proteins and impurities, washed with ethanol to remove salts, and
finally the RNA was eluted with Elution Buffer.
18
MAGBEADS FASTDNA/RNA KIT FOR VIRUS
SIMPLE AND RELIABLE: Total viral nucleic acid from cell-free/low-cell content biological samples such
as body fluids, serum, plasma as well as tissue homogenates and culture supernatant.
FLEXIBLE: manual workflow or easily adaptable to automated platforms
HIGH QUALITY : nucleic acids are suitable for a variety of downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Magnetic Beads 192 Preps 116571192
The Magbeads FastDNA/RNA Kit for Virus is based on the purification method of high binding magnetic particles.
The sample is lysed and digested with Protease. DNA/RNA is released into the lysate. After addition of magnetic
particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities
such as proteins will be removed without adsorption. The adsorbed particles are washed with washing solution
to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA is eluted with
Nuclease Free Water.
Need more information on our RNA Kits?
Visit our webpage
Would like to try one of our RNA Kits?
Request your FREE sample!
19
MP BIOMEDICALS
AMERICAS: 800.854.0530 | custserv.na@mpbio.com www.mpbio.com
EUROPE: 00800.7777.9999 | custserv.eur@mpbio.com
APAC: +65 6775.0008 | custserv.ap@mpbio.com
20
Purification Kits
Perfect Solutions for your most Precious Samp les
Complete Kits including Specialized Lysing Matrix Tubes to make
Biological Sample Lysis Easy
High RNA yield, purity and integrity
20 YEARS OF EXPERTISE IN SAMPLE PREPARATION AND MOLECULAR BIOLOGY
MP BIOMEDICALS1 www.mpbio.com
INTRODUCTION
Explore our Portfolio of RNA Extraction Kits to Find the Right Solution for your
Application
MP Biomedicals supplies a complete range of organic-based, spin column-based as well as magnetic beads-based
extraction kits for the isolation of high quality DNA-free RNA.
Efficient lysis of the starting material and simultaneous inactivation of endogenous RNases is achieved by mixing
the sample with the lysis buffer in a 2mL tube containing lysing matrix beads that is then processed in a FastPrep
homogenizer or equivalent bead beater.
After a centrifugation step to pellet the lysing matrix beads and cell debris, the RNA is purified with the reagents
supplied in the kit using a ready-to-use protocol.
Total RNA isolated with MP Biomedicals kits has a high purity and is ready-to-use for a broad panel of downstream
applications: Next-Gen sequencing, RT-PCR , cDNA-library, TaqMan® analysis and array technologies,
Northern Blot, RNA dot blots and in vitro translation.
The FastPrep System
A Complete Solution for Sample Preparation
Sample Holders Lysing Matrix Tubes DNA/RNA Extraction Kits
Any Size, Any Temperature 16 combinaison of beads to Ready-to-Use Protocols
grind any sample for DNA, RNA and Protein
Isolation from any Sample!
POWERFUL Thorough grinding and lysis of the most difficult samples in just a few seconds.
FLEXIBLE Easily interchangeable sample holders to process any sample size (2mL, 4.5mL, 15mL, 50mL
tubes, 250mL flasks and 96 well plates) at cryogenic or room temperature.
COMPLETE SOLUTION Large number of ready-to-use Lysing Matrix compositions and FastPrep Kits for
DNA, RNA and protein purification of any sample.
2
RNA ISOLATION AND PURIFICATION KIT
SELECTION GUIDE
Sample Type Product Name Cat. No. Technology Prep No. Page
FastRNA Pro Green Kit 116045050 Organic Extraction 50 Preps 4
Human, animal and
SPINeasy RNA Kit for
plant tissues, cultured 116543050 Silica Spin Columns 50 Preps 5
Tissue
cells
Magbeads FastRNA Kit 116572096 Magnetic Beads 96 Preps 18
Formalin-fixed paraffin-
Magbeads FastRNA Kit 192
embedded tissues and 116573192 Magnetic Beads 18
for FFPE Preps
cells
Gram positive and FastRNA Pro Blue Kit 116025050 Organic extraction 50 Preps 7
gram negative bacteria,
bacterial spores, virus SPINeasy RNA Kit for
116541050 Silica spin columns 50 Preps 8
Bacteria
Bacteria Magbeads FastRNA Kit 116572096 Magnetic beads 96 Preps 18
Magbeads FastDNA/ 192
Virus 116571792 Magnetic beads 19
RNA Kit for virus Preps
FastRNA Pro Red Kit 116035050 Organic extraction 50 Preps 10
Yeast, fungi FastRNA SPIN Kit for
116030050 Silica spin columns 50 Preps 11
Yeast
Microorganisms and
other specimens FastRNA Pro Soil
116070050 Organic extraction 50 Preps 12
found in soil and other Direct Kit
and purification
environmental samples
with silica spin
(extraction from the FastRNA Pro Soil
116075050 filters
sample without pre- 50 Preps 13
Indirect Kit
wash step)
Any sample DNA/
SPINeasy DNA/RNA/
RNA and Protein from a 116544050 Silica spin columns 50 Preps 14
Protein All-In-One Kit
Single Sample
Agarose and
Silica and spin 200
polyacrylamide gels as RNaid SPIN Kit 111107200 15
filters Preps
well as any solution
3
ANIMAL & PLANT TISSUES
FASTRNA PRO GREEN KIT
EFFICIENT: Lysis of human, animal, and plant tissues with
Lysing Matrix D tubes
CONSISTENT YIELDS: RNA isolation with the single-
reagent RNAPro solution
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
REF
Organic Extraction 50 Preps 116045050
The FastRNA Pro Green Kit is optimized for purification of total RNA from human, animal and plant samples. Add
50-300 mg of tissue to a 2 ml tube containing Lysing Matrix D and the proprietary RNApro Solution. After one 40
second run in the FastPrep Instrument, total RNA is isolated via chloroform extraction and ethanol precipitation.
Tail Liver Brain Tail Liver Blood
0.24-9.5 kb
50- 2000bp
Kidney Ear RNA marker Kidney Brain DNA marker.
Figure 1 - Rat total RNA extracted with the FastRNA Pro Green Kit. Figure 2 - RT-PCR of actin genes from rat RNA extracted with the
Approximately 2% of the total RNA isolated from 100 mg frozen FastRNA Pro Green Kit. 20% of the RT-PCR reaction was loaded on
tissue was loaded on to a 1.2% denaturing agarose gel (1X MOPS). a 1.2% agarose gel (0.5X TAE).
4
ANIMAL & PLANT TISSUES
SPINEASY RNA KIT FOR TISSUE
EFFICIENT : Lysis of any human, animal, plant tissue or cell
culture sample with Lysing Matrix A tubes
CONVENIENT : Silica spin column method for an easy
extraction process
HIGH-QUALITY : RNA is ready-to-use for a variety of
downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116543050
SPINeasy RNA Kit for Tissue is a silica-membrane spin-column kit that enables quick and convenient
purification of total RNA from various human/ animal tissues, plant tissues and tissue cultures.
The use of Lysing Matrix A tubes enables highly efficient lysis of tissue samples within seconds. Purified RNA is
of high quality and immediately ready for RT-PCR and other downstream applications.
Starting
Sample Extraction Results
Amount (mg)
Concentration A A
260/280 260/230
Rabbit Liver 21.4 626.46 2.08 2.19
Rabbit Spleen 14.0 310.53 2.04 2.26
Rabbit Kidney 22.3 237.88 2.06 2.21
Rabbit Lung 21.4 159.24 2.01 2.26
Rabbit Heart 22.5 54.56 2.01 2.82
Ginkgo Biloba Leaf 51.3 132.93 2.09 2.21
Prunus Davidiana
52.7 382.08 2.07 1.94
Leaf (wild peach)
Cherry Leaf 48.5 294.06 2.06 1.91
Begonia Leaf 52.0 368.66 2.06 1.94
Tomato Leaf 52.2 663.82 2.10 2.28
Peanut Leaf 70.2 898.92 2.10 2.31
Potato Leaf 52.4 491.20 2.14 2.30
5
ANIMAL & PLANT TISSUES
Liver Spleen Kidney Lung Heart DNA Marker Ginkgo Prunus Cherry Begonia Tomato Peanut Potato
Biloba Davidian a Leaf Leaf Leaf Leaf Leaf
Leaf Leaf
Figure 1: RNA extracted from various samples using SPINeasy RNA Kit for Tissue, analyzed using agarose gel
electrophoresis.
DNA Liver Spleen Kidney Hear t Lung Negative Ginkgo Prunus Begonia Peanut Potato Negative
Marker Control Biloba Davidiana Leaf Leaf Leaf Control
( rabbit ?-actin) Leaf Leaf (Plant 18S)
Cherry Tomato
Leaf Leaf
Figure 2: RT-PCR amplification of RNA extracted from various samples using SPINeasy RNA Kit for Tissue.
Build your Molecular Biology Workflow
PCR Enzymes, Mastermixes and Kits
END-POINT PCR
REAL TIME PCR
REVERSE TRANSCRIPTION
DEOXYNUCLEOTIDES
6
BACTERIA
FASTRNA PRO BLUE KIT
EFFICIENT: Lysis of any gram-positive and gram-negative
bacteria with Lysing Matrix B tubes
CONSISTENT YIELDS: RNA isolation with the single-reagent
RNAPro solution
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
REF
Organic Extraction 50 Preps 116025050
The FastRNA Pro Blue Kit is optimized for purification of total RNA from both gram positive and gram negative
bacteria. Add a pellet of up to 1010cells in suspension to a 2 ml tube containing Lysing Matrix B and the proprietary
RNApro Solution. After one 40 second run in the FastPrep Instrument, total RNA is isolated via chloroform
extraction and ethanol precipitation.
Achieve Optimal Lysing Performance with FastPrep Lysing Matrix Tubes
Optimal cell disruption for any sample
Size of the beads and composition optimized according to the sample
No cross contamination with closed Lysing Matrix tubes
Available in 2ml, 4.5ml, 15ml , 50ml tubes or 96 well plates
Fit any high-speed bead-beating machines
7
BACTERIA
SPINEASY RNA KIT FOR BACTERIA
EFFICIENT: Lysis of any gram-positive and gram-negative bacteria
with Lysing Matrix B tubes
CONVENIENT: Silica spin column method for an easy extraction
process
HIGH-QUALITY: RNA is ready-to-use for a variety of downstream
applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116541050
SPINeasy RNA Kit for Bacteria is a silica-membrane spin-column kit that enables quick and convenient purification
of total RNA from gram-positive and gram-negative bacteria.A specially formulated RNASS solution that stabilizes
and protects RNA in bacteria samples is included in the kit.
The use of Lysing Matrix B tubes in combination with a FastPrep instrument enables highly efficient lysis of
bacterial samples within seconds.
Total RNA of high quality is purified within 40 minutes and is immediately available for RT-PCR and other
downstream applications.
Sample Starting Amount (mg) Extraction Results
Concentration A A
260/280 260/230
E. Coli Pellet from 3 mL overnight culture 763.59 2.10 2.26
S. Aureus Pellet from 3 mL overnight culture 349.47 2.13 2.04
E. Coli Negative
E. Coli S. Aureus
S. Aureus
Figure 1: Total RNA extracted from bacteria Figure 2: RT-PCR amplification of RNA extracted
samples using SPINeasy RNA Kit for Bacteria, from bacteria samples using SPINeasy RNA Kit for
analyzed using agarose gel electrophoresis. M: Bacteria. M: DNA marker; Lane 1: E. coli; Lane 2: S.
DNA marker; Lane 1: E. coli; Lane 2: S. aureus. aureus; Lane 3: Negative control.
8
CASE STUDYCASE STUDY - BACTERIA
SARS-COV-2 RNA IN WASTEWATER
FastRNA Pro Blue Kit
Cat. No. 116025050
Shelesh Agrawal, Laura Orschler & Susanne Lackner.
Sci Rep 11, 5372 (2021).
A Proven-Method for the Isolation of pure RNA from Wastewater ready-to-use for monitoring the
SARS-CoV-2 RNA concentration in sewage
Long-term monitoring of SARS-CoV-2 RNA in wastewater of the Frankfurt metropolitan area in Southern Germany.
EFFICIENT MECHANICAL LYSIS OF VIRAL PARTICLES: with Lysing Matrix B tubes containing 0.1mm silica beads
included in the kit
HIGH YIELDS OF PURE RNA: with the RNApro reagent developed to protect RNA from degradation by RNases and
to isolate RNA via a phenol chloroform extraction method
SUCCESSFUL RT-qPCR ANALYSIS: extracted viral RNA was used as a template in a multiplex assay targeting the
N- , S- and ORF1ab viral genes.
Concentrations of SARS-CoV-2 RNA in the influent of the SARS-CoV-2 load as sum of the two WWTP influents
WasteWater Treatment Plant (WWTP) of Niederrad as determined as analyzed with RT-qPCR in comparison to the positive
by real-time qPCR tested COVID-19 cases in the city of Frankfurt am Main.
9
YEAST & FUNGI
FASTRNA PRO RED KIT
EFFICIENT: Lysis of yeast and fungi with Lysing Matrix C tubes
CONSISTENT YIELDS: RNA isolation with the single-reagent
RNAPro solution
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
REF
Organic Extraction 50 Preps 116035050
The FastRNA Pro Red Kit is optimized for purification of total RNA from yeast and fungi. Add a pellet of up to 1010
cells in suspension to a 2 ml tube containing Lysing Matrix C and the proprietary RNAPro Solution. After one 40
second run in the FastPrep Instrument, total RNA is isolated via chloroform extraction and ethanol precipitation.
Figure 1 - Yeast and fungal total RNA extracted with Figure 2 - RT-PCR of actin genes from yeast
the FastRNA Pro Red Kit. Approximately 2% of the total and fungal RNA extracted with the FastRNA
RNA isolated from 100 mg tissue or 1010 cells was loaded Pro Red Kit. 20% of the RT-PCR reaction was
onto a 1.2% denaturing agarose gel (1X MOPS). Lane 1: loaded on a 1.2% agarose gel (0.5X TAE).
S. pombe; Lane 2: S. cerevisiae; Lane 3: P. pastoris; Lane Lane 1: S. cerevisiae; Lane 2: P. pastoris;
4: C. albicans; Lane 5: common mushroom; Lane 6: small Lane 3: C. albicans; Lane 4: 50-2000bp DNA
common mushroom; Lane 7: 0.24-9.5kb RNA Ladder. marker.
10
YEAST & FUNGI
FASTRNA SPIN KIT FOR YEAST
EFFICIENT: Lysis of yeast and fungi with Lysing Matrix Y tubes
RAPID AND REPRODUCIBLE: Purification of intact high quality
total RNA with the use of specialized spin columns
HIGH-QUALITY: RNA is ready-to-use for a variety of
downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116030050
The FastRNA SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast
strains and fungi in approximately 30 minutes using Lysing Matrix Y tubes for cell lysis and spin columns for the
purification process. High quality total RNA is eluted with RNase-free water. Both large and small RNA species
are recovered with this protocol.
Encapsulated Media
• NO WEIGHING • NO DUST • NO CLEANUP • NO SMELL
Hundreds of formulation for bacteria and yeast
Capsule format eliminates weighing, dust and cleanup
simply drop capsules in water and autoclave
11
SOIL & ENVIRONMENTAL SAMPLES
The FastRNA Pro Soil-Direct and Indirect kits are designed to efficiently isolate total RNA from organic material
found in soil samples, soil supernatants or any other environmental samples.
The direct method consists of extracting nucleic acid from microorganisms and other biological specimens
directly from soil. The indirect method utilizes an initial separation of microorganisms and other biological
specimens from the soil followed by lysis of the organisms and RNA purification. FastRNA Pro Soil kits purify
RNA in a process that removes humic substances and other inhibitors and efficiently inactivates cellular RNases
during homogenization to prevent RNA degradation. Purified RNA is thus suitable for RT-PCR analysis and other
downstream applications.
FASTRNA PRO SOIL DIRECT KIT
RAPID & EFFICIENT: Thorough sample lysis in seconds of any soil
and environmental sample with Lysing Matrix E tubes
HIGH PURITY: 2 levels of purification to remove efficiently humic
acids and other inhibitors
RELIABLE: Cellular RNases are inactivated during homogenization to
prevent RNA degradation
REF
Organic Extraction 50 Preps 116070050
The FastRNA Pro Soil-Direct is designed to efficiently isolate total RNA from organic material found in soil
samples. Following processing with Lysing Matrix E in the FastPrep Instrument, samples are extracted with
chloroform and cleaned with RNAMATRIX and other proprietary reagents. Purified RNA is suitable for RT-PCR
analysis, Next-Gen Sequencing and other downstream applications.
Total RNA extracted from Soil Samples with the FastRNA®
Pro Soil-Direct Kit.Approximately 15% of the total RNA
isolated from 0.5 g of four different soil samples was loaded
on to a 0.8% agarose gel.
Soil #1 Soil #5 Soil #10 Soil #11
1 kb ladder
12
SOIL & ENVIRONMENTAL SAMPLES
RT-PCR of Fungal Gene from Total RNA Isolated from
Soil Samples with the FastRNA® Pro Soil-Direct Kit.
Approximately 40% of the RT-PCR reaction was loaded on
to a 0.8% agarose gel.
150bp Soil #1 Soil #5 Soil #10 Soil #11
-2kb marker
FASTRNA PRO SOIL INDIRECT KIT
CLEAN: Initial separation of microorganisms and other
biological specimens from soil
RAPID & EFFICIENT: Thorough sample lysis in seconds of
any microorganism present in soil and environmental sample
with Lysing Matrix E tubes
CONSISTENT: Efficient removal of PCR inhibitors and
inactivation of cellular RNases during the homogenization
step
REF
Organic Extraction 50 Preps 116075050
The FastRNA Pro Soil Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil
samples or soil supernatants. Following processing with Lysing Matrix E in the FastPrep Instrument, samples
are extracted with chloroform and cleaned with RNAMATRIX and other proprietary reagents. Purified RNA is
suitable for RT-PCR analysis, Next-Gen Sequencing and other downstream applications.
Total RNA extracted from Soil Samples with the FastRNA® RT-PCR of Fungal Gene from Total RNA Isolated from
Pro Soil-Indirect Kit. Approximately 15% of the total RNA Soil Samples with the FastRNA® Pro Soil-Indirect Kit.
isolated from four different soil samples was loaded on to a Approximately 40% of the RT-PCR reaction was loaded
0.8% agarose gel. Lane 1: 1kb ladder, Lane 2: RNA from 5 g on to a 0.8% agarose gel. Lane 1: 150bp – 2kb marker,
Soil #1, Lane 3: RNA from 10 g Soil #2, Lane 4: RNA from 5 Lane 2: Soil #1, Lane 3: Soil #2, Lane 4: Soil #7, Lane 5:
g Soil #7, Lane 5: RNA from 5 g Soil #10. Soil #10.
13
SIMULTANEOUS PURIFICATION OF RNA, DNA AND PROTEINS
SPINEASY DNA/RNA/PROTEINS ALL-IN-ONE KIT
RAPID: Isolate DNA/RNA/Protein from a single sample in one hour
EFFICIENT: Lysis of any resistant sample with Lysing Matrix A tubes
CONVENIENT: Easy-to-use silica spin column method for the DNA
and RNA extraction process
HIGH-QUALITY: DNA, RNA and proteins are suitable for a variety
of downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Silica Spin Columns 50 Preps 116544050
SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica-membrane spin columns
to isolate DNA, RNA and protein components from the same sample.
After an efficient cell lysis by bead beating with Lysing Matrix A tubes, DNA is adsorbed onto the first spin
column, while the flow-through is collected and used for RNA purification. The second spin column captures
RNA and the flow-through from this step is used for protein extraction.
DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted.
In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed and
resuspended.
Each molecular component is then immediately available for their respective downstream applications.
Starting
Sample Extraction Results
Amount (mg)
DNA RNA Protein
Yield A A Yield A A Yield
(µg/mg sample) 260/280 260/230 (µg/mg sample) 260/280 260/230 (µg/mg sample)
Kidney 17.7 1.28 1.83 2.42 1.30 2.04 2.23 14.12
Spleen 12.4 1.81 1.84 2.45 1.81 2.04 2.22 20.81
Liver 17.0 1.09 1.84 2.89 3.48 2.07 2.17 10.16
14
Figure 1 (left): (A) DNA; (B) RNA; (C) protein extracted from each animal Figure 2 (right): (A) PCR amplification of DNA and (B) RT-PCR
tissue using SPINeasy DNA/RNA/Protein All-In-One Kit, analyzed using amplification of RNA extracted from various samples using SPINeasy
gel electrophoresis. M: DNA marker; Lane 1: Kidney; Lane 2: Spleen; Lane DNA/RNA/Protein All-In-One Kit. M: DNA marker; Lane 1: Kidney;
3: Liver. Lane 2: Spleen; Lane 3: Liver; Lane 4: Negative control.
RNA CLEAN UP
RNAID SPIN KIT
MAXIMUM RECOVERY: >90% recovery of RNA fragments of up to 10 kb
RAPID: 3 easy steps - bind, wash and elute
HIGH QUALITY: RNA is ready-to-use for any downstream application including reverse transcription
and Next-Gen Sequencing
REF
Silica and Spin Filters 200 Preps 111107200
The RNaid SPIN Kit contains all the reagents and materials necessary for the isolation and purification of RNA
from agarose and polyacrylamide gels as well as from any solution in 20 minutes.
This kit in combination with the FastDNA SPIN Kit for Soil (Cat N°: 116560200) is also used to co-extract
RNA and DNA from the same soil sample (protocol described in the article : Tournier E. et al, MethodsX. 2015 Mar
27;2:182-91).
15
CASE STUDY
SIMULTANEOUS ISOLATION OF DNA AND RNA
FROM SOIL
Combined use of:
FastPrep-24 instrument
Cat. No. 116004500
FastDNA SPIN Kit for Soil
Cat. No. 116560200
RNaid SPIN Kit
Cat. No. 111107200
Tournier, E.; Amenc, L.; Pablo, A. L.; Legname, E.; Blanchart
, E.; Plassard, C.; Robin, A.; Bernard, L. MethodsX.
2015, 182.
A comparison of the composition of microbial communities based on co-extraction of both DNA and RNA provides insight
into the ecology of populations, provided that DNA and RNA are subjected to the same extraction bias. This study describes
the isolation of soil nucleic acids with simultaneous extraction and purification of DNA and RNA following a cascade scheme
involving the FastDNA SPIN Kit for Soil, the FastPrep instrument and the RNaid Kit and avoiding the use of harmful solvents
Reproducible: The coextraction protocol was optimized on a sandy clay loam soil. Yields of 26.7 µg of purified DNA
and 4.5 µg of purified RNA per gram of soil were obtained with very good repeatability for both DNA and RNA.
Reliable: The four tropical soils from Madagascar were extractable and gave various DNA and RNA recovery yields
sufficiently concentrated to be further analyzed by any other molecular technique.
Efficient:The protocol was efficient on different tropical soils, including Andosol, where their high contents of clays,
including poorly crystalline clays, and Fe and Al oxides typically make the nucleic acid extraction more difficult.
DNA (black) and RNA (light grey) extraction yields and their
respective 95% confidence intervals (alpha 0.05, 3 replicates) from
4 tropical soils, sampled in Madagascar and characterized by
different textures, mineralogies, metal and carbon contents.
16
AUTOMATED NUCLEIC ACID PURIFICATION PLATFORM
MPURE-32 SYSTEM
FULLY AUTOMATED MAGNETIC BEAD-BASED
NUCLEIC ACID EXTRACTION SYSTEM
Fast and Consistent Results
Superior Purity and Yield
High-efficiency and stability
MPure-32™ aNAP System is a fully automatic platform for
magnetic reagent assays. It can process up to 32 samples
simultaneously within a short period of time (around 40
to 60 minutes). Still achieves Scientific and Diagnostic
requirement with safe operating procedure.
17
MAGBEADS FASTRNA KIT
QUICK AND SIMPLE: RNA extraction from animal and plant tissues, cells in culture as well as bacteria
and yeast in one hour
FLEXIBLE: Manual workflow or easily adaptable to automated platforms
HIGH QUALITY : RNA is ready for all downstream applications including RT-PCR and Next-Gen
sequencing
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Magnetic Beads 96 Preps 116572096
The Magbeads FastRNA Kit combines the speed and efficiency of silica-based technology with the convenient
handling of magnetic particles for purification of total RNA.
Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the
particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and
DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA
is eluted in RNase-free water.
MAGBEADS FASTRNA KIT FOR FFPE
SIMPLE AND RELIABLE: RNA extraction from tissue, cells and blood. A deparaffinization Solution is
included in the kit for an efficient paraffin removal.
FLEXIBLE: manual workflow or easily adaptable to automated platforms
HIGH QUALITY : RNA is ready for all downstream applications including RT-PCR and Next-Gen
sequencing
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Magnetic Beads 192 Preps 116573192
The Magbeads FastRNA Kit for FFPE is based on the purification method of high binding magnetic particles. The
sample is lysed and digested. RNA is released into the lysate.
After addition of magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic
particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were
washed with washing buffer to remove the proteins and impurities, washed with ethanol to remove salts, and
finally the RNA was eluted with Elution Buffer.
18
MAGBEADS FASTDNA/RNA KIT FOR VIRUS
SIMPLE AND RELIABLE: Total viral nucleic acid from cell-free/low-cell content biological samples such
as body fluids, serum, plasma as well as tissue homogenates and culture supernatant.
FLEXIBLE: manual workflow or easily adaptable to automated platforms
HIGH QUALITY : nucleic acids are suitable for a variety of downstream applications
ENVIRONMENTALLY FRIENDLY: No toxic chemicals
REF
Magnetic Beads 192 Preps 116571192
The Magbeads FastDNA/RNA Kit for Virus is based on the purification method of high binding magnetic particles.
The sample is lysed and digested with Protease. DNA/RNA is released into the lysate. After addition of magnetic
particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities
such as proteins will be removed without adsorption. The adsorbed particles are washed with washing solution
to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA is eluted with
Nuclease Free Water.
Need more information on our RNA Kits?
Visit our webpage
Would like to try one of our RNA Kits?
Request your FREE sample!
19
MP BIOMEDICALS
AMERICAS: 800.854.0530 | custserv.na@mpbio.com www.mpbio.com
EUROPE: 00800.7777.9999 | custserv.eur@mpbio.com
APAC: +65 6775.0008 | custserv.ap@mpbio.com
20
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Date d'upload du document :
vendredi 4 octobre 2024