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Sample Preparation-Brochure
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FastPrep® System
A Complete Solution for Your Sample Preparation
Higher Yields
Consistent Quality
Super Fast
The Ultimate Grinding and Cell Lysis Technology Delivering the Most
DNA, RNA and Proteins from Any Sample Type in Just Seconds!
Fast and easy, everytime.
www.mpbio.com
Brochure Index
Sample Preparation
Sample Preparation 3
Presentation & Workflow ................................................................................................................................................................................................................. 3
Protocol .................................................................................................................................................................................................................................................... 4
FastPrep®Instruments 5
FastPrep® Instruments ....................................................................................................................................................................................................................... 6
FastPrep-24® 5G ................................................................................................................................................................................................................................... 7
FastPrep-24® Classic .......................................................................................................................................................................................................................... 8
FastPrep-96® .......................................................................................................................................................................................................................................... 9
SuperFastPrep-2® .............................................................................................................................................................................................................................. 10
FastPrep® Tube Holders 11
FastPrep® Tube Holders .................................................................................................................................................................................................................. 12
Ambient Tube Holders ..................................................................................................................................................................................................................... 13
Cryogenic Tube Holders .................................................................................................................................................................................................................. 15
Metal Tube Holders ........................................................................................................................................................................................................................... 16
High Capacity Tube Holders .......................................................................................................................................................................................................... 17
Locking Systems ................................................................................................................................................................................................................................. 19
Frequently Asked Questions ......................................................................................................................................................................................................... 20
FastPrep® Lysing Matrix 21
Standard Lysing Matrix Tubes ....................................................................................................................................................................................................... 22
Lysing Matrix Tubes Selection Guide ......................................................................................................................................................................................... 23
Metal Lysing Matrix Tubes ............................................................................................................................................................................................................. 27
Automated Nucleic Acid Purification Systems 29
MPure-12TM .......................................................................................................................................................................................................................................... 30
MPure-12TM Nucleic Acid Purification Kits ............................................................................................................................................................................. 31
MPure-32TM .......................................................................................................................................................................................................................................... 33
MPure-96TM .......................................................................................................................................................................................................................................... 34
Nucleic Acid and Protein Purification Kits 35
FastPrep® DNA and RNA Kits ....................................................................................................................................................................................................... 36
DNA Isolation & Purification Kits 37
Multi-samples: Eukaryotes & Prokaryotes .............................................................................................................................................................................. 37
Bodily Fluids ........................................................................................................................................................................................................................................ 39
Soil, Environmental & Fecal Samples ......................................................................................................................................................................................... 42
Water Samples .................................................................................................................................................................................................................................... 46
Plant Specimens ................................................................................................................................................................................................................................. 47
RNA Isolation & Purification Kits 48
Animal & Plant Tissues .................................................................................................................................................................................................................... 48
Bacteria .................................................................................................................................................................................................................................................. 51
Yeast & Fungi ....................................................................................................................................................................................................................................... 52
Soil, Environmental & Fecal Samples ......................................................................................................................................................................................... 53
Protein Isolation & Purification Kits 54
FastProtein™ Matrix - Bacteria and Yeast ............................................................................................................................................................................... 54
Simultaneous Isolation & Purification of DNA, RNA, and Proteins ............................................................................................................ 55
Products Index ...................................................................................................................................................................................................................... 56
www.mpbio.com 2
Sample Preparation
Presentation & Workflow
MP Biomedicals, the leader in sample preparation for more than 25 years provides a complete range of high-quality products for
all steps of your research experiments. From lysis and extraction through purification of DNA, RNA, and proteins, we offer the
best solutions to achieve excellent and reliable results for your applications.
MP Biomedicals: Dependability Delivered
MP Biomedicals offers a complete sample preparation solution with
FastPrep® instruments, Lysing Matrix tubes and extraction kits.
Utilizing bead-beating technology, the FastPrep® system provides
complete lysis of virtually any sample type and is suitable for a
variety of applications from soil analysis to forensics. Mechanical
sample homogenization with the FastPrep® system enables the
isolation of DNA, RNA, proteins, and other small molecules while
eliminating the need for chemicals or enzymes, which can inhibit
downstream processes.
Our Workflow
Fast Grinding & Lysis Isolation & Purification of
DNA, RNA & Proteins
FastPrep®
Instruments
Automated
FastPrep® Purification
Tube Holders
Manual
Lysing Matrix
Purification
FastPrep® homogenizers pulverize samples through simultaneous beating of specialized Lysing Matrix beads.
Interchangeable sample holders allow unique flexibility in terms of tube size (2 mL to 250 mL, as well as 96 deep well plates)
and temperature (ambient or cryogenic conditions). FastPrep® systems can quickly and efficiently process routine and
resistant samples, including plant, root, soil, waste water, skin, tissue, seeds, and feces. FastPrep® instruments, combined
with the widest selection of industry leading Lysing Matrix materials and complete isolation kits, offer a complete solution
for processing even the most difficult samples.
www.mpbio.com 3
Sample Preparation
Protocol
MP Biomedicals, the leader in sample preparation, provides a complete range of high-quality products for all steps of your research
experiments. From lysis and extraction through purification of DNA, RNA and proteins, we offer the best solutions to achieve
excellent and reliable results for your applications. FastPrep® systems deliver high yields of DNA, RNA, and proteins from even
the most resistant sample types in 40 seconds or less.
MP Biomedicals: Dependability Delivered
FastPrep® instruments, Lysing Matrix tubes, and kits work together to deliver 1. Prepare lysis tube
rapid, consistent, and efficient lysis and homogenization, resulting in high yields
of purified nucleic acid or protein. A benchtop instrument utilizing bead-beating
technology, the FastPrep® provides complete and quantitative lysis of difficult Buffer Sample
and routine samples and is suitable in all applications that require grinding, from kit
lysing, or homogenization.
Examples of sample types include, but are not limited to:
Plant – Stems, roots, leaves, buds, flowers, fruits, and seeds
Animal – Animal and human samples, including bone, tumors, and skin
Soil – Eubacterial spores and endospores, gram-positive bacteria, yeast, algae, nematodes, 2. Process with FastPrep®
fungi, clay, sandy, silty, peaty, chalky, and loamy soil samples instruments
Bacteria – Gram-positive, gram-negative, eubacterial spores, and endospores
Feces – Complex fecal matrices
Yeast – Cells and spores
MP Biomedicals offers genomic DNA and total RNA extraction and purification
kits and reagents that are optimized to provide maximum yield, purity and
integrity from any sample.
3. Centrifuge to pellet
cell debris
MP Biomedicals extraction and purification kits offer the following benefits:
Rapid and reproducible sample lysis and purification.
Closed Lysing Matrix tubes to prevent cross-contamination.
Increased yields of high-quality DNA and RNA.
Integrity and size of DNA and RNA are retained.
Ready-to-use nucleic acids for downstream applications.
4. Transfer cleared lysate
DNA Protein RNA
Gel Purify
analysis
www.mpbio.com 4
FastPrep® Instruments
Crush, Homogenize, Lyse Efficiently. Nothing Resists FastPrep®
Plants Animals Soil Yeast Bacteria Feces
Super FastPrep-2® FastPrep-24® Classic FastPrep-24® 5G FastPrep-96®
Cat. N°: 116012500 Cat. N°: 116004500 Cat. N°:116005500 Cat. N°: 116010500
Low Throughput / High-Throughput /
Low volumes High volumes
The FastPrep® family is a comprehensive laboratory solution that optimizes the lysis, grinding,
or homogenization process from virtually any sample type. Mechanical lysis disrupts cells and
tissues for the isolation of DNA, RNA, proteins, metabolites, and other small molecules, and
eliminates the need for chemicals, enzymes, and detergents, which can inhibit some downstream
processes.
www.mpbio.com 5
FastPrep® Instruments
Find the Right Model
Achieve complete lysis of virtually any sample type with FastPrep® instruments. Known for consistent performance that generates
reproducible results, the FastPrep® bead beating system is carefully engineered to maximize mechanical tissue homogenization.
As a result, you can efficiently isolate high-quality DNA, RNA, and proteins without the need for chemicals or enzymes, which
might inhibit downstream processes.
Super FastPrep-2® FastPrep-24® Classic FastPrep-24® 5G FastPrep-96®
Cat. N°: 116012500 Cat. N°: 116004500 Cat. N°: 116005500 Cat. N°: 116010500
High-Throughput,
Description High-Performance, Handheld Bench-Top Bead Advanced Bench-Top Bead
High-Performance Bead
Field Grinding System Beating System Beating System
Beating System
Sample capacity 2 Up to 48 Up to 48 Up to 192
Adapters No Interchangeable Interchangeable Interchangeable
Tube Compatibility 2 mL, 4.5 mL, 5 mL, 15 mL, 2 mL, 4.5 mL, 5mL, 15 mL, 2 mL, 4.5 mL, 15 mL, 50 mL, 96
2 mL
50 mL 50 mL plate, 250 mL
Cryogenic Lysis No Ye s Ye s Yes with cryo adapters
Interface Manual-button LCD/Membrane keyboard Touch Screen VFD/Industrial buttons
Pre-Defined Protocols No No 72 No
User Defined Protocols No 5 12 No
Min Speed 600 CPM* 4.0 m/s 4.0 m/s 800 RPM**
Max Speed 4,800 CPM* 6.5 m/s 10.0 m/s 1,800 RPM**
Acceleration Ø 500 G < 2 sec to max < 2 sec to max < 2 sec to max
Deceleration NA < 2 sec to stop < 2 sec to stop < 2 sec to stop
Motion Reciprocating Figure 8 Tridimentional Figure 8 Tridimentional Linear Vertical
Typical Lysis Time (s) 5 s 40 s 20 s 40 s
Dimensions 117 mm (H) x 86 mm (W) x 330 465 mm (H) x 332 mm (W) x 490 mm (H) x 385 mm (W) x 700 mm (H) x 440 mm (W) x
mm (L) 437 mm (L) 472 mm (L) 660 mm (L)
Weight 2.2 kg 17.5 kg (45 lb) 23.6 kg (52 lb) 49 kg (108 lb)
Loudness < 90 dB 70 dB < 70 dB < 65 dB
Power Requirements 90 - 240 V for battery charger, 110 VAC/60 Hz; 230 VAC/50 110 VAC/60 Hz; 230 VAC/50 110 VAC/60 Hz, 5.2A; 220
cordeless operation Hz, 500 W Hz, 500 W VAC/50 Hz, 2.6A
110/230V Switch Battery Loader Spec Automatic Automatic Reversible Manually
* CPM Cycle Per Minute; ** RPM Revolution Per Minute
The FastPrep® instruments are versatile sample disruption and grinding devices that provides the ultimate in speed and
performance for the lysis and homogenizing of biological or crushing of inorganic samples.
www.mpbio.com 6
FastPrep-24® 5G MOST VERSATILE:
Often Imitated, Never Replicated.
Most Advanced Lysis, Homogenization, and Grinding System Applicable for
Genomics, Proteomics, or Other Chemical Studies and Analysis.
The FastPrep-24®5G instrument is a versatile sample disruption device
that provides the ultimate in speed and performance for the lysis of
biological or grinding of inorganic samples.
A completely self-contained system, the FastPrep-24® 5G instrument
eliminates the risk of cross-contamination and time-consuming clean-up
associated with manual lysis methods.
Samples and buffers are simply added to a Lysing Matrix tube containing
specialized Lysing Matrix particles. Select your sample type from the
recommended programs menu, push run, and in 40 seconds or less, your
samples are completely lysed. The FastPrep-24® 5G also allows for up to
12 custom assays to be manually programmed and saved.
QuickPrep™ 3 tube holder included.
Capacity: 24 x 2 mL.
Cat. No: 116005500
Specifications
Interface Touch Screen Interface
Programmable Assays Up to 12 Manual Assays Saved to Memory
Pre-Defined Assay 72 Pre-Defined and Optimized Assay Programs
Run Time 1 to 120 seconds in 1-second Increments
Speed Range 4 to 10 m/sec in 0.5 m/sec Increments
Cycles 1 to 9 Cycles
Temperature Operating Range 2 to 48 °C (35 to 118 °F)
Data Export Via USB Cable
Dimensions Height: 490 mm; Base: 385 mm (W) x 472 mm (L) (Elliptic Shape)
Weight 23.6 kg (52 lb)
Mp biomedical: Dependability Delivered
Power Requirement 110 VAC/60 Hz; 230 VAC/50 Hz, 500 W
The heartbeat of the FastPrep-24® 5G is a Powerful: Thorough grinding of most difficult samples in just a few seconds.
microprocessor control interfaced to a touch
Intuitive: Interactive user-friendly interface and touchscreen with more than 60
screen display. The large 7-inch HD monitor pre-programmed protocols.
allows assay parameters to be set with the touch
of a button. Hi-def graphics and intuitive software Complete: High number of ready-to-use Lysing Matrix tubes and a wide range of
make programming the FastPrep-24® 5G fast purification kits for DNA, RNA, and proteins.
and simple, while high-tech exterior graphics Flexible:Easily interchangeable adapters to process any sample size (2 mL, 4.5 mL, 5 mL,
add to the sleek and sophisticated design of the 15 mL or 50 mL tubes) at cryogenic or room temperature.
instrument.
www.mpbio.com 7
FastPrep-24® Classic
Bench-Top Bead Beating Lysis System
Grind, homogenize, and lyse any sample in 40 seconds or less.
The FastPrep-24® Classic instrument is a high-speed benchtop
homogenizer offering the ultimate in speed and performance for the lysis
of biological samples. Simultaneous homogenization of up to 24 samples
takes place within 40 seconds.
Cat. No: 116004500
Consistent results
Reliable
Flexible
Affordable
8,500 citations
QuickPrep™ 1 tube holder included.
High yields Capacity: 24 x 2 mL.
Power to homogenize resistant samples with ease
Interchangeable sample holders for flexibility in sample size and cryogenic lysis capability:
24 x 2 mL • 48 x 2 mL • 24 x 4.5 mL • 16 x 5 mL • 6 x 15 mL • 12 x 15 mL • 2 x 50 mL
High reproducibility with Eliminate cross-contamination Save up to 5 pre-set Complete purification
precise setting of lysis time with single-use Lysing Matrix speed/time parameters kits with Lysing Matrix
and speed tubes available
Specifications
Run Time 1 - 60 seconds in 1-second Increments
Speed 4.0 - 6.5 m/sec in 0.5 m/sec Increments
Acceleration < 2 Seconds to Maximum Speed
Deceleration < 2 Seconds to Stop
Temperature
5 - 35 °C (41 - 95 °F)
Operating Range
Dimensions Height: 465 mm, Oval Base: 332 mm (W) x 437 mm (L)
Weight 17.9 kg (45 lb)
Power Requirements 110/230 VAC, 50/60 Hz, 500 W
www.mpbio.com 8
FastPrep-96®
High-Throughput Bead Beating Grinder and Lysis System
Grinding, lysis, and homogenization with the high-throughput FastPrep-96® system.
MP Biomedicals FastPrep-96® is a versatile high-speed homogenizer
offering the ultimate in high-throughput sample preparation.
Developed for resistant samples, the FastPrep-96® instrument uses a
high-speed linear motion to disrupt thoroughly any tissues and cells
in just seconds through the simultaneous beating of specialized Lysing
Matrix beads on the sample material.
This high-performance system saves hours of work during the sample
preparation stage and allows for the purification of high yields of intact
DNA, RNA, proteins, and other metabolites.
Cat. No: 116010500 Dual Plate tube holder included.
Capacity: 2 x 96 deep well plates.
High-Throughput Bead Beating Grinder and Lysis System
High-Throughput – Process up to 192 samples simultaneously in
2 x 96 deep well plates
Exceptional Versatility – Interchangeable sample holders available:
96 x 2 mL tubes • 48 x 4.5 mL tubes • 20 x 15 mL tubes
8 x 50 mL tubes • 2 x 250 mL bottles
Fast Processing Speed – up to 1,800 Oscillations/min
True Linear Motion – Eliminates the need to re-orient plates mid-cycle
Efficiently Lyse:
Animal Bacteria Yeast Fungi Plants
Specifications
Controls Programmable Run Time and Speed; Display Readout
1 - 360 seconds; 1 - 60 seconds in 1-second Increments
Time Range
60 - 360 seconds in 30-second Increments
Speed Range 800 - 1,800 Revolutions Per Minutes (RPM)
Speed Range
Programmable in 200 RPM Increments
Acceleration < 2 Seconds to Maximum Speed
Deceleration < 2 Seconds to Stop
Weight 49 kg (108 lb)
Power Requirement 110 VAC/60 Hz, 5.2 A
Overvoltage Category II 220 VAC/50 Hz, 2.6 A
Temperature Operating Range 2 - 48 °C (36 - 118 °F)
Dimensions Height: 700 mm; Base: 440 mm (W) x 660 mm (L)
www.mpbio.com 9
SuperFastPrep-2®
High-Performance, Handheld Field Lysis System
Lysis, homogenization, and grinding at your fingertips.
The SuperFastPrep2® is a compact, cordless, hand-held sample disruption
instrument designed to quickly and efficiently homogenize, grind, or lyse
biological and environmental samples. It can be used at remote locations
and in most weather conditions for the immediate collection, processing,
and preservation of samples.
SuperFastPrep-2® uses a unique mechanism mounted on a rotary tool
designed to operate at 600 to 4,800 cycles per minute with a fully charged
battery. The self-contained device holds two standard 2 mL screw cap
Lysing Matrix tubes optimized for a wide selection of sample types.
Included with the unit: a rotary handle, two lithium-ion rechargeable
batteries, a charging station, a voltage converter, and a protective
carrying case.
Cat. No: 116012500
Omni-directional motion with the highest speed.
Handheld system for lab and field use.
Compatible with all FastPrep® 2 mL Lysing Matrix tubes.
Specifications
Power Requirements 90 - 240 V for Battery Charger, Cordless Operation
Dimensions 117 mm (H), 86 mm (W), 330 mm (L)
Weight 2.2 kg
Number of Tubes per Run 2
Size of Tube 2 mL Tubes
Maximum Speed 4,800 Cycles Per Minute (CPM)
Minimum Speed 600 Cycles Per Minutes (CPM)
Type of Motion Reciprocating
Peak-to-Peak Amplitude of Motion 38 mm
Sound at 1ft. 90 dB at 3,300 CPM
Typical Run Time 2 - 15 seconds at 3,300 CPM
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FastPrep® Tube Holders
Tube Holders for FastPrep® Systems are Flexible, Interchangeable, and Available for Ambient,
Cryogenic or Infectious Sample Types
Ambient Cryogenic Metal High
Temperature Temperature volumes
Standard tube
holders for ambient
temperature work
Cryogenic tube holders
for temperature Metal tube holders
sensitive sample - sterilizable
High capacity tube holders for
large sample volume
MP Biomedicals offers the widest selection of tube holders to best meet your needs in sample
preparation. Our tube holders allow for sample sizes ranging from 2 mL to 250 mL tube size and
are built for durability in ambient and cryogenic conditions.
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FastPrep® Tube Holders
Adapter for FastPrep® Systems are Flexible, Interchangeable, and Available for Ambient or
Cryogenic or Infectious Sample Types
Application Tube Size Tube Capacity Name Page
2 mL tubes 24 QuickPrep™ 13
2 mL tubes 48 HiPrep™ 13
4.5 mL tubes 24 TallPrep™ 13
Standard tube holders
for ambient 5 mL tubes 16 MidiPrep™ 14
temperature work
15 mL tubes 12 TeenPrep™ 14
50 mL tubes 2 BigPrep™ 14
2 mL tubes 24 Metal QuickPrep™ 16
Low & Medium
Throughput 5 mL tubes 18 Metal MidiPrep™ 16
FastPrep® 24s Metal tube holders 15 mL tubes 12 Metal TeenPrep™ 16
- sterilizable
50 mL tubes 2 Metal BigPrep™ 16
2 mL tubes 24 CoolPrep™ 15
Temperature 15 mL tubes 6 CoolTeenPrep™ 15
sensitive
sample 50 mL tubes 2 CoolBigPrep™ 15
96 deep well plates 2 x 96 Dual Plate™ 17
2mL tubes 96 QuickFlex™ 17
4.5 mL tubes 48 TallFlex™ 17
Standard ambient
temperature 15 mL tubes 24 TeenFlex™ 18
High-Throughput
& Volume 50 mL tubes 8 BigFlex™ 18
FastPrep® 96 250 mL bottles 2 LargeFlex™ 18
www.mpbio.comwww.mpbio.com 12
Ambient Tube Holders
Ambient Temperature Sample Grinding with FastPrep-24® and FastPrep-24® 5G Instruments
Ambient temperature tube holders for all your classic and routine grinding.
MP Biomedicals offers the widest selection of tube holders to best meet
your needs in sample lysis and grinding.
Our tube holders allow for sample sizes ranging from 2 mL to 250 mL and are
built for durability in ambient and cryogenic conditions.
QuickPrep™ 1
Cat. N°: 116002512
Capacity: 24 x 2 mL Tubes
QuickPrep™ 3
Cat. N°: 116005512
Capacity: 24 x 2 mL Tubes
HiPrep™
Cat. N°: 116002527
Capacity: 48 x 2 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
TallPrep™
Cat. N°: 116002540
Capacity: 24 x 4.5 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
www.mpbio.comwww.mpbio.com 13
Ambient Tube Holders
Ambient Temperature Sample Grinding with FastPrep-24® and FastPrep-24® 5G Instruments
Ambient temperature tube holders for all your classic and routine grinding.
MidiPrep™
Cat. N°: 116002557
Capacity: 16 x 5 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
TeenPrep™
Cat. N°: 116002526
Capacity: 12 x 15 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
BigPrep™
Cat. N°: 116002525
Capacity: 2 x 50 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
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Cryogenic Tube Holders
Temperature Sensitive Sample Grinding with FastPrep-24® and FastPrep-24® 5G Instruments
Tube holders for extraction of any sensitive or thermally unstable biological compound.
Cryogenic temperature tube holders for FastPrep-24® Classic and
FastPrep-24® 5G instruments. During mechanical lysis, the temperature
within the tube can increase and cause damage to the molecules in your
sample.
Protects thermosensitive molecules from heat degradation with cooling
chamber.
Prevents the increase of sample temperature during the homogenization
process by maintaining sample temperature at negative temperature.
Ensures a highly effective grinding process of any sample, even the most
elastic, by making them brittle.
CoolPrep™
Cat. N°: 116002528
Capacity: 24 x 2 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
Cool TeenPrep™
Cat. N°: 116002530
Capacity: 6 x 15 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
Cool BigPrep™
Cat. N°: 116002531
Capacity: 2 x 50 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
www.mpbio.comwww.mpbio.com 15
Metal Tube Holders
Infectious Sample Grinding with FastPrep-24® Classic and FastPrep-24® 5G Instruments
All metal sample holders are ideally suited for work with highly infectious,
pathogenic, or other biologically hazardous samples.
They withstand temperatures up to 450 °C, allowing for sterilization by
pyrolysis or autoclaving. Pathogens, including bacteria, viruses, fungi,
parasites, viroids, and prions, can be effectively eliminated.
All metal sample holders are also safe to use with most laboratory detergents
and sterilization solutions, ensuring easy care and maintenance.
MetalQuickPrep™
Cat. N°: 116002545
Capacity: 24 x 2 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
MetalMidiPrep™
Cat. N°: 116002544
Capacity: 18 x 5 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
MetalTeenPrep™
Cat. N°: 116002546
Capacity: 12 x 15 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
MetalBigPrep™
Cat. N°: 116002547
Capacity: 2 x 50 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
www.mpbio.com 16
High Capacity Tube Holders
Ambient Temperature Sample Grinding with FastPrep-96® Instruments
FastPrep-96® offers the widest variety of sample holders (2 x 96 deep well
plates, 96 x 2 mL, 48 x 4.5 mL, 24 x 15 mL, 8 x 50 mL and 2 x 250 mL bottles)
and a simple, accurate, closed loop control of lysing power and speed.
All this and more make the FastPrep-96® the perfect solution for all of your
high volume sample preparation needs.
Dual Plate™
Cat. N°: 119696168
Capacity: 2 x 96 Deep Well Plates
QuickFlex™
Cat. N°: 116010570
Capacity: 96 x 2 mL Tubes
TallFlex™
Cat. N°: 116010580
Capacity: 48 x 4.5 mL Tubes
www.mpbio.com 17
High Capacity Tube Holders
Sample Holders for FastPrep-96® Instrument
Ambient temperature tube holders for high-throughput and large volume grinding.
TeenFlex™
Cat. N°: 116010560
Capacity: 20 x 15 mL Tubes
BigFlex™
Cat. N°: 116010550
Capacity: 8 x 50 mL Tubes
LargeFlex™
Cat. N°: 116010590
Capacity: 2 x 250 mL Bottles
ConeFlex™ Adapter
For use of FastPrep-24® tube holders
on FastPrep-96®
Cat. N°: 116010595
For all FastPrep-24® tube holders
www.mpbio.com 18
Locking Systems
Locking Systems for FastPrep-24® Classic & FastPrep-24® 5G Instruments.
MP Biomedicals offers the widest selection of sample holders to best meet
your needs in sample preparation. Our sample holders allow for sample sizes
ranging from 2 mL to 250 mL tube size and are built for durability in ambient
and cryogenic conditions.
Ratchet Nut for FastPrep-24® Classic
Cat. No: 116004510
For FastPrep-24® Classic
Replacement tightening spoked button used to secure
adapters on FastPrep-24® Classic.
Blue Clickmaster, Ratchet Nut
Cat. N°: 116004525
For FastPrep-24® Classic or FastPrep-24® 5G
Accessory for FastPrep-24®Classic and FastPrep-24®
5G instruments. Securing knob used to maintain the
tube holder adapters on the central axis or 3 step
assembly.
Due to its high torque, the Ratchet Nut is particularly
recommended for the heaviest adapters, and especially
for metal tube holders.
Camlock
Cat. N°: 116005588
For FastPrep-24® 5G Instrument
Locking system for the FastPrep-24® 5G instrument.
Modeled after the bike tire quick release, the familiar
mechanism allows:
Rapid sample exchange.
Strong grip.
Uniform clamping force.
Ease-of-use.
www.mpbio.com 19
Frequently Asked Questions
Should I balance the tubes when using a FastPrep® instrument?
No. The FastPrep® is not a centrifuge and you do not need to balance the tubes on adapters.
However, if you do not fill the tube holders completely, avoid having all your tubes in one location.
Place your tubes evenly over all the holes in your adapter. To ensure better stability for the top parts
of the adapters, it is important that the adapter rests on more than two points. We recommend
using these with a minimum of 4 tubes. Use empty tubes if necessary .
There is nothing on the display of the FastPrep-24® Classic or
FastPrep-24® 5G, but it is powered and turned on?
Each device is shipped with the ‘emergency stop’ engaged for safety purposes and will not run until
the emergency stop is disengaged. Disengage the ‘emergency stop’ by turning clockwise until it
pops out. EMERGENCY
STOP
How do I convert from the m/s used to describe FastPrep® motion to
the RPM measurements used by other bead beating systems?
The RPM value used by other bead beating systems refers to rotations per minute. However,
FastPrep® instruments do not use a rotating motion but rather a tridimensional motion. The
FastPrep®’s m/s refers to the distance traveled by the sample or adapter bottom during one second.
To convert from rpm to m/s, compare the number of turns carried out by the axis of the engine
against the meter per second below.
• Motor speed at 4 m/s is equivalent to between 3,400 to 4,100 RPM.
• Motor speed at 6.5 m/s is equivalent to between 3,700 to 4,500 RPM.
Note that this conversion depends on the adapter used and the distance from tubes to the axis of
the FastPrep®.
Can I use and leave my FastPrep® in a cold room at 4 °C for a long
period of time? 4 °C
The operating temperature range for the FastPrep® Instruments is from 2 – 48 °C so storing at
4 °C is not a problem. MP Biomedicals has thoroughly tested using and storing a FastPrep®in a
cold room and have confirmed with other customers. However, it was built to be used at room
temperature so there is a risk of condensed water negatively affecting the motor oil.
Where can I have more technical information or European declaration
of conformity.
Contact us to have more
On our website www.mpbio.com/eu/life-sciences/sample-preparation-0/fastprep-bead-beating-systems informations:
you can download user manual and find all specification. techsup.eur@mpbio.com
For certificates, service manual or any other information about MP Biomedicals FastPrep® please www.mpbio.com
address your request through website to our European technical support team. They will be happy
to help .
www.mpbio.com 20
FastPrep® Lysing Matrix
Experience Unmatched Versatility
For your toughest and most difficult sample types
Plants Animals Soil Yeast and Bacteria Feces
Fungi
2 mL 4.5 mL 15 mL 50 mL 250 mL 96 deep well
Lysing Matrix tubes Lysing Matrix tubes Lysing Matrix tubes Lysing Matrix tubes Lysing Matrix bottles Lysing Matrix plates
FastPrep® Lysing Matrices make difficult-to-lyse samples easy. No matter how tough or resistant
your samples are FastPrep® Lysing Matrix tubes will effectively disrupt cell walls, providing the
highest yields of nucleic acids and proteins in a matter of seconds. All Lysing Matrix tubes fit any
high-speed bead-beating homogenizers. MP Biomedicals offers a wide variety of lysing bead
mixes to fit all sample types and applications.
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Standard Lysing Matrix Tubes
Ready-to-use for all your Sample Types
Achieve optimal lysing performance with FastPrep® Lysing Matrix tubes.
FastPrep® Lysing Matrices are critical components of the FastPrep® sample preparation system.
Lysing Matrix tubes come in 16 unique matrix compositions to ensure leak and contamination-free homogenization of any
sample type.
Optimal cell disruption of any sample:The size of the beads and their composition are
optimized according to the sample and the downstream application.
A solution for any application: Unmatched versatility with 16 matrix compositions
available in 2 mL, 4.5 mL, 15 mL, 50 mL, 250 mL bottles or 96-well plates.
High reproducibility: Lysing Matrix tubes contain highly controlled size and quantity of
Lysing Matrix particles and are certified DNase & RNase free.
No cross-contamination: Single-use closed Lysing Matrix tubes.
A gold standard for Life Science applications validated with + 9,000 publications: They
fit any high-speed bead-beating machines.
Because Size Matters
All Lysing Matrix tubes offered come in every size, from 2 mL to 250 mL as well as 96 deep well plates.
Up to Up to Up to Up to Up to
150 mg 500 mg 1 g 3 g 50 g
96 deep well plates 2 mL tube 4.5 mL tube 15 mL tube 50 mL tube 250 mL bottle
Description Pack Size
2 mL 50, 100 and 500 x 2 mL tubes
N
R a
s
4.5 mL 25, 50 and 100 x 4.5 mL tubes /
e e
s F
a R
N E
15 mL 25 and 50 x 15 mL tubes D E
50 mL 10, 50 and 100 x 50 mL tubes
C D
250 mL 10 x 250 mL bottles E
R E
I
T I F
96 deep well plates 1 and 10 x 96 deep well plates
www.mpbio.com 22
Lysing Matrix Tubes Selection Guide
Find the Lysing Matrix Tailored to your Application
Sample Type Lysing Matrix
Animal & Human Tissues A B C D E F G H I J K M S SS Y Z
Lung, Breast, Kidney, Heart,
Soft Tissues Intestine, Muscle,
Spleen, Liver, Brain
Skin
Nail
Tail & Ear
Vascular Tissue
Unique Hair
Samples
Bone
Tumor
Mammalian Cell
Infected Tissue (isolation of
viruses or virus)
Microorganisms A B C D E F G H I J K M S SS Y Z
Bacteria (gram + and -)
Yeast & Mold
Bacterial & Fungal Spore
Algae
Virus
Environmental Samples A B C D E F G H I J K M S SS Y Z
Soil, Marine Sediment,
Rhizosphere, Manure,
Compost, Sludge, Feces,
Wastewater
Plant Tissues A B C D E F G H I J K M S SS Y Z
Leaf
Seed
Root
Needle
Wood
Stem & Flower
Insects & Worms A B C D E F G H I J K M S SS Y Z
Tick & Fly
Nematode
Bee & Mosquito
www.mpbio.com 23
Lysing Matrix Tubes
Experience Unmatched Versatility
Achieve optimal lysing performance with FastPrep® Lysing Matrix tubes.
Lysing Matrix A Lysing Matrix B Lysing Matrix C
Composition: Garnet matrix and one Composition: 0.1 mm silica spheres Composition: 1 mm silica spheres
6.35 mm ceramic sphere
All sample types Animal Fungi Bacteria Spores Yeast Fungi Algae
Bacteria Yeast Plants
Lysing Matrix D Lysing Matrix E Lysing Matrix F
Composition: 1.4 mm ceramic spheres Composition: 1.4 mm ceramic spheres, Composition: 1.6 mm aluminum oxide
0.1 mm silica spheres and 4 mm glass bead particles and 1.6 mm silicon carbide particles
Animal tissues Plants tissues Soil Fecal Sludge Pollen Animal tissues Plants tissues
Cultured cells Wastewater Environmental water Bacteria Mold & fungi
Lysing Matrix G Lysing Matrix H Lysing Matrix I
Composition: 1.6 mm silicon carbide Composition: 2 mm glass beads and 2 mm Composition: 2 mm yellow zirconia beads
particles and 2 mm glass beads yellow zirconium oxide beads and one 4 mm ceramic sphere
Animal Plants Ancient & dried samples
Animal tissues Plants tissues Animal Plants Coral emulsions
Very dense soil and clay Bacterial aggregates
Yeast Fungi Molds & fungi Fixed samples
Whole insects, tickes & fleas Wood & seeds
www.mpbio.com 24
Lysing Matrix Tubes
Experience Unmatched Versatility
Achieve optimal lysing performance with FastP
A Complete Solution for Your Sample Preparation
Higher Yields
Consistent Quality
Super Fast
The Ultimate Grinding and Cell Lysis Technology Delivering the Most
DNA, RNA and Proteins from Any Sample Type in Just Seconds!
Fast and easy, everytime.
www.mpbio.com
Brochure Index
Sample Preparation
Sample Preparation 3
Presentation & Workflow ................................................................................................................................................................................................................. 3
Protocol .................................................................................................................................................................................................................................................... 4
FastPrep®Instruments 5
FastPrep® Instruments ....................................................................................................................................................................................................................... 6
FastPrep-24® 5G ................................................................................................................................................................................................................................... 7
FastPrep-24® Classic .......................................................................................................................................................................................................................... 8
FastPrep-96® .......................................................................................................................................................................................................................................... 9
SuperFastPrep-2® .............................................................................................................................................................................................................................. 10
FastPrep® Tube Holders 11
FastPrep® Tube Holders .................................................................................................................................................................................................................. 12
Ambient Tube Holders ..................................................................................................................................................................................................................... 13
Cryogenic Tube Holders .................................................................................................................................................................................................................. 15
Metal Tube Holders ........................................................................................................................................................................................................................... 16
High Capacity Tube Holders .......................................................................................................................................................................................................... 17
Locking Systems ................................................................................................................................................................................................................................. 19
Frequently Asked Questions ......................................................................................................................................................................................................... 20
FastPrep® Lysing Matrix 21
Standard Lysing Matrix Tubes ....................................................................................................................................................................................................... 22
Lysing Matrix Tubes Selection Guide ......................................................................................................................................................................................... 23
Metal Lysing Matrix Tubes ............................................................................................................................................................................................................. 27
Automated Nucleic Acid Purification Systems 29
MPure-12TM .......................................................................................................................................................................................................................................... 30
MPure-12TM Nucleic Acid Purification Kits ............................................................................................................................................................................. 31
MPure-32TM .......................................................................................................................................................................................................................................... 33
MPure-96TM .......................................................................................................................................................................................................................................... 34
Nucleic Acid and Protein Purification Kits 35
FastPrep® DNA and RNA Kits ....................................................................................................................................................................................................... 36
DNA Isolation & Purification Kits 37
Multi-samples: Eukaryotes & Prokaryotes .............................................................................................................................................................................. 37
Bodily Fluids ........................................................................................................................................................................................................................................ 39
Soil, Environmental & Fecal Samples ......................................................................................................................................................................................... 42
Water Samples .................................................................................................................................................................................................................................... 46
Plant Specimens ................................................................................................................................................................................................................................. 47
RNA Isolation & Purification Kits 48
Animal & Plant Tissues .................................................................................................................................................................................................................... 48
Bacteria .................................................................................................................................................................................................................................................. 51
Yeast & Fungi ....................................................................................................................................................................................................................................... 52
Soil, Environmental & Fecal Samples ......................................................................................................................................................................................... 53
Protein Isolation & Purification Kits 54
FastProtein™ Matrix - Bacteria and Yeast ............................................................................................................................................................................... 54
Simultaneous Isolation & Purification of DNA, RNA, and Proteins ............................................................................................................ 55
Products Index ...................................................................................................................................................................................................................... 56
www.mpbio.com 2
Sample Preparation
Presentation & Workflow
MP Biomedicals, the leader in sample preparation for more than 25 years provides a complete range of high-quality products for
all steps of your research experiments. From lysis and extraction through purification of DNA, RNA, and proteins, we offer the
best solutions to achieve excellent and reliable results for your applications.
MP Biomedicals: Dependability Delivered
MP Biomedicals offers a complete sample preparation solution with
FastPrep® instruments, Lysing Matrix tubes and extraction kits.
Utilizing bead-beating technology, the FastPrep® system provides
complete lysis of virtually any sample type and is suitable for a
variety of applications from soil analysis to forensics. Mechanical
sample homogenization with the FastPrep® system enables the
isolation of DNA, RNA, proteins, and other small molecules while
eliminating the need for chemicals or enzymes, which can inhibit
downstream processes.
Our Workflow
Fast Grinding & Lysis Isolation & Purification of
DNA, RNA & Proteins
FastPrep®
Instruments
Automated
FastPrep® Purification
Tube Holders
Manual
Lysing Matrix
Purification
FastPrep® homogenizers pulverize samples through simultaneous beating of specialized Lysing Matrix beads.
Interchangeable sample holders allow unique flexibility in terms of tube size (2 mL to 250 mL, as well as 96 deep well plates)
and temperature (ambient or cryogenic conditions). FastPrep® systems can quickly and efficiently process routine and
resistant samples, including plant, root, soil, waste water, skin, tissue, seeds, and feces. FastPrep® instruments, combined
with the widest selection of industry leading Lysing Matrix materials and complete isolation kits, offer a complete solution
for processing even the most difficult samples.
www.mpbio.com 3
Sample Preparation
Protocol
MP Biomedicals, the leader in sample preparation, provides a complete range of high-quality products for all steps of your research
experiments. From lysis and extraction through purification of DNA, RNA and proteins, we offer the best solutions to achieve
excellent and reliable results for your applications. FastPrep® systems deliver high yields of DNA, RNA, and proteins from even
the most resistant sample types in 40 seconds or less.
MP Biomedicals: Dependability Delivered
FastPrep® instruments, Lysing Matrix tubes, and kits work together to deliver 1. Prepare lysis tube
rapid, consistent, and efficient lysis and homogenization, resulting in high yields
of purified nucleic acid or protein. A benchtop instrument utilizing bead-beating
technology, the FastPrep® provides complete and quantitative lysis of difficult Buffer Sample
and routine samples and is suitable in all applications that require grinding, from kit
lysing, or homogenization.
Examples of sample types include, but are not limited to:
Plant – Stems, roots, leaves, buds, flowers, fruits, and seeds
Animal – Animal and human samples, including bone, tumors, and skin
Soil – Eubacterial spores and endospores, gram-positive bacteria, yeast, algae, nematodes, 2. Process with FastPrep®
fungi, clay, sandy, silty, peaty, chalky, and loamy soil samples instruments
Bacteria – Gram-positive, gram-negative, eubacterial spores, and endospores
Feces – Complex fecal matrices
Yeast – Cells and spores
MP Biomedicals offers genomic DNA and total RNA extraction and purification
kits and reagents that are optimized to provide maximum yield, purity and
integrity from any sample.
3. Centrifuge to pellet
cell debris
MP Biomedicals extraction and purification kits offer the following benefits:
Rapid and reproducible sample lysis and purification.
Closed Lysing Matrix tubes to prevent cross-contamination.
Increased yields of high-quality DNA and RNA.
Integrity and size of DNA and RNA are retained.
Ready-to-use nucleic acids for downstream applications.
4. Transfer cleared lysate
DNA Protein RNA
Gel Purify
analysis
www.mpbio.com 4
FastPrep® Instruments
Crush, Homogenize, Lyse Efficiently. Nothing Resists FastPrep®
Plants Animals Soil Yeast Bacteria Feces
Super FastPrep-2® FastPrep-24® Classic FastPrep-24® 5G FastPrep-96®
Cat. N°: 116012500 Cat. N°: 116004500 Cat. N°:116005500 Cat. N°: 116010500
Low Throughput / High-Throughput /
Low volumes High volumes
The FastPrep® family is a comprehensive laboratory solution that optimizes the lysis, grinding,
or homogenization process from virtually any sample type. Mechanical lysis disrupts cells and
tissues for the isolation of DNA, RNA, proteins, metabolites, and other small molecules, and
eliminates the need for chemicals, enzymes, and detergents, which can inhibit some downstream
processes.
www.mpbio.com 5
FastPrep® Instruments
Find the Right Model
Achieve complete lysis of virtually any sample type with FastPrep® instruments. Known for consistent performance that generates
reproducible results, the FastPrep® bead beating system is carefully engineered to maximize mechanical tissue homogenization.
As a result, you can efficiently isolate high-quality DNA, RNA, and proteins without the need for chemicals or enzymes, which
might inhibit downstream processes.
Super FastPrep-2® FastPrep-24® Classic FastPrep-24® 5G FastPrep-96®
Cat. N°: 116012500 Cat. N°: 116004500 Cat. N°: 116005500 Cat. N°: 116010500
High-Throughput,
Description High-Performance, Handheld Bench-Top Bead Advanced Bench-Top Bead
High-Performance Bead
Field Grinding System Beating System Beating System
Beating System
Sample capacity 2 Up to 48 Up to 48 Up to 192
Adapters No Interchangeable Interchangeable Interchangeable
Tube Compatibility 2 mL, 4.5 mL, 5 mL, 15 mL, 2 mL, 4.5 mL, 5mL, 15 mL, 2 mL, 4.5 mL, 15 mL, 50 mL, 96
2 mL
50 mL 50 mL plate, 250 mL
Cryogenic Lysis No Ye s Ye s Yes with cryo adapters
Interface Manual-button LCD/Membrane keyboard Touch Screen VFD/Industrial buttons
Pre-Defined Protocols No No 72 No
User Defined Protocols No 5 12 No
Min Speed 600 CPM* 4.0 m/s 4.0 m/s 800 RPM**
Max Speed 4,800 CPM* 6.5 m/s 10.0 m/s 1,800 RPM**
Acceleration Ø 500 G < 2 sec to max < 2 sec to max < 2 sec to max
Deceleration NA < 2 sec to stop < 2 sec to stop < 2 sec to stop
Motion Reciprocating Figure 8 Tridimentional Figure 8 Tridimentional Linear Vertical
Typical Lysis Time (s) 5 s 40 s 20 s 40 s
Dimensions 117 mm (H) x 86 mm (W) x 330 465 mm (H) x 332 mm (W) x 490 mm (H) x 385 mm (W) x 700 mm (H) x 440 mm (W) x
mm (L) 437 mm (L) 472 mm (L) 660 mm (L)
Weight 2.2 kg 17.5 kg (45 lb) 23.6 kg (52 lb) 49 kg (108 lb)
Loudness < 90 dB 70 dB < 70 dB < 65 dB
Power Requirements 90 - 240 V for battery charger, 110 VAC/60 Hz; 230 VAC/50 110 VAC/60 Hz; 230 VAC/50 110 VAC/60 Hz, 5.2A; 220
cordeless operation Hz, 500 W Hz, 500 W VAC/50 Hz, 2.6A
110/230V Switch Battery Loader Spec Automatic Automatic Reversible Manually
* CPM Cycle Per Minute; ** RPM Revolution Per Minute
The FastPrep® instruments are versatile sample disruption and grinding devices that provides the ultimate in speed and
performance for the lysis and homogenizing of biological or crushing of inorganic samples.
www.mpbio.com 6
FastPrep-24® 5G MOST VERSATILE:
Often Imitated, Never Replicated.
Most Advanced Lysis, Homogenization, and Grinding System Applicable for
Genomics, Proteomics, or Other Chemical Studies and Analysis.
The FastPrep-24®5G instrument is a versatile sample disruption device
that provides the ultimate in speed and performance for the lysis of
biological or grinding of inorganic samples.
A completely self-contained system, the FastPrep-24® 5G instrument
eliminates the risk of cross-contamination and time-consuming clean-up
associated with manual lysis methods.
Samples and buffers are simply added to a Lysing Matrix tube containing
specialized Lysing Matrix particles. Select your sample type from the
recommended programs menu, push run, and in 40 seconds or less, your
samples are completely lysed. The FastPrep-24® 5G also allows for up to
12 custom assays to be manually programmed and saved.
QuickPrep™ 3 tube holder included.
Capacity: 24 x 2 mL.
Cat. No: 116005500
Specifications
Interface Touch Screen Interface
Programmable Assays Up to 12 Manual Assays Saved to Memory
Pre-Defined Assay 72 Pre-Defined and Optimized Assay Programs
Run Time 1 to 120 seconds in 1-second Increments
Speed Range 4 to 10 m/sec in 0.5 m/sec Increments
Cycles 1 to 9 Cycles
Temperature Operating Range 2 to 48 °C (35 to 118 °F)
Data Export Via USB Cable
Dimensions Height: 490 mm; Base: 385 mm (W) x 472 mm (L) (Elliptic Shape)
Weight 23.6 kg (52 lb)
Mp biomedical: Dependability Delivered
Power Requirement 110 VAC/60 Hz; 230 VAC/50 Hz, 500 W
The heartbeat of the FastPrep-24® 5G is a Powerful: Thorough grinding of most difficult samples in just a few seconds.
microprocessor control interfaced to a touch
Intuitive: Interactive user-friendly interface and touchscreen with more than 60
screen display. The large 7-inch HD monitor pre-programmed protocols.
allows assay parameters to be set with the touch
of a button. Hi-def graphics and intuitive software Complete: High number of ready-to-use Lysing Matrix tubes and a wide range of
make programming the FastPrep-24® 5G fast purification kits for DNA, RNA, and proteins.
and simple, while high-tech exterior graphics Flexible:Easily interchangeable adapters to process any sample size (2 mL, 4.5 mL, 5 mL,
add to the sleek and sophisticated design of the 15 mL or 50 mL tubes) at cryogenic or room temperature.
instrument.
www.mpbio.com 7
FastPrep-24® Classic
Bench-Top Bead Beating Lysis System
Grind, homogenize, and lyse any sample in 40 seconds or less.
The FastPrep-24® Classic instrument is a high-speed benchtop
homogenizer offering the ultimate in speed and performance for the lysis
of biological samples. Simultaneous homogenization of up to 24 samples
takes place within 40 seconds.
Cat. No: 116004500
Consistent results
Reliable
Flexible
Affordable
8,500 citations
QuickPrep™ 1 tube holder included.
High yields Capacity: 24 x 2 mL.
Power to homogenize resistant samples with ease
Interchangeable sample holders for flexibility in sample size and cryogenic lysis capability:
24 x 2 mL • 48 x 2 mL • 24 x 4.5 mL • 16 x 5 mL • 6 x 15 mL • 12 x 15 mL • 2 x 50 mL
High reproducibility with Eliminate cross-contamination Save up to 5 pre-set Complete purification
precise setting of lysis time with single-use Lysing Matrix speed/time parameters kits with Lysing Matrix
and speed tubes available
Specifications
Run Time 1 - 60 seconds in 1-second Increments
Speed 4.0 - 6.5 m/sec in 0.5 m/sec Increments
Acceleration < 2 Seconds to Maximum Speed
Deceleration < 2 Seconds to Stop
Temperature
5 - 35 °C (41 - 95 °F)
Operating Range
Dimensions Height: 465 mm, Oval Base: 332 mm (W) x 437 mm (L)
Weight 17.9 kg (45 lb)
Power Requirements 110/230 VAC, 50/60 Hz, 500 W
www.mpbio.com 8
FastPrep-96®
High-Throughput Bead Beating Grinder and Lysis System
Grinding, lysis, and homogenization with the high-throughput FastPrep-96® system.
MP Biomedicals FastPrep-96® is a versatile high-speed homogenizer
offering the ultimate in high-throughput sample preparation.
Developed for resistant samples, the FastPrep-96® instrument uses a
high-speed linear motion to disrupt thoroughly any tissues and cells
in just seconds through the simultaneous beating of specialized Lysing
Matrix beads on the sample material.
This high-performance system saves hours of work during the sample
preparation stage and allows for the purification of high yields of intact
DNA, RNA, proteins, and other metabolites.
Cat. No: 116010500 Dual Plate tube holder included.
Capacity: 2 x 96 deep well plates.
High-Throughput Bead Beating Grinder and Lysis System
High-Throughput – Process up to 192 samples simultaneously in
2 x 96 deep well plates
Exceptional Versatility – Interchangeable sample holders available:
96 x 2 mL tubes • 48 x 4.5 mL tubes • 20 x 15 mL tubes
8 x 50 mL tubes • 2 x 250 mL bottles
Fast Processing Speed – up to 1,800 Oscillations/min
True Linear Motion – Eliminates the need to re-orient plates mid-cycle
Efficiently Lyse:
Animal Bacteria Yeast Fungi Plants
Specifications
Controls Programmable Run Time and Speed; Display Readout
1 - 360 seconds; 1 - 60 seconds in 1-second Increments
Time Range
60 - 360 seconds in 30-second Increments
Speed Range 800 - 1,800 Revolutions Per Minutes (RPM)
Speed Range
Programmable in 200 RPM Increments
Acceleration < 2 Seconds to Maximum Speed
Deceleration < 2 Seconds to Stop
Weight 49 kg (108 lb)
Power Requirement 110 VAC/60 Hz, 5.2 A
Overvoltage Category II 220 VAC/50 Hz, 2.6 A
Temperature Operating Range 2 - 48 °C (36 - 118 °F)
Dimensions Height: 700 mm; Base: 440 mm (W) x 660 mm (L)
www.mpbio.com 9
SuperFastPrep-2®
High-Performance, Handheld Field Lysis System
Lysis, homogenization, and grinding at your fingertips.
The SuperFastPrep2® is a compact, cordless, hand-held sample disruption
instrument designed to quickly and efficiently homogenize, grind, or lyse
biological and environmental samples. It can be used at remote locations
and in most weather conditions for the immediate collection, processing,
and preservation of samples.
SuperFastPrep-2® uses a unique mechanism mounted on a rotary tool
designed to operate at 600 to 4,800 cycles per minute with a fully charged
battery. The self-contained device holds two standard 2 mL screw cap
Lysing Matrix tubes optimized for a wide selection of sample types.
Included with the unit: a rotary handle, two lithium-ion rechargeable
batteries, a charging station, a voltage converter, and a protective
carrying case.
Cat. No: 116012500
Omni-directional motion with the highest speed.
Handheld system for lab and field use.
Compatible with all FastPrep® 2 mL Lysing Matrix tubes.
Specifications
Power Requirements 90 - 240 V for Battery Charger, Cordless Operation
Dimensions 117 mm (H), 86 mm (W), 330 mm (L)
Weight 2.2 kg
Number of Tubes per Run 2
Size of Tube 2 mL Tubes
Maximum Speed 4,800 Cycles Per Minute (CPM)
Minimum Speed 600 Cycles Per Minutes (CPM)
Type of Motion Reciprocating
Peak-to-Peak Amplitude of Motion 38 mm
Sound at 1ft. 90 dB at 3,300 CPM
Typical Run Time 2 - 15 seconds at 3,300 CPM
www.mpbio.com 10
FastPrep® Tube Holders
Tube Holders for FastPrep® Systems are Flexible, Interchangeable, and Available for Ambient,
Cryogenic or Infectious Sample Types
Ambient Cryogenic Metal High
Temperature Temperature volumes
Standard tube
holders for ambient
temperature work
Cryogenic tube holders
for temperature Metal tube holders
sensitive sample - sterilizable
High capacity tube holders for
large sample volume
MP Biomedicals offers the widest selection of tube holders to best meet your needs in sample
preparation. Our tube holders allow for sample sizes ranging from 2 mL to 250 mL tube size and
are built for durability in ambient and cryogenic conditions.
www.mpbio.comwww.mpbio.com 11
FastPrep® Tube Holders
Adapter for FastPrep® Systems are Flexible, Interchangeable, and Available for Ambient or
Cryogenic or Infectious Sample Types
Application Tube Size Tube Capacity Name Page
2 mL tubes 24 QuickPrep™ 13
2 mL tubes 48 HiPrep™ 13
4.5 mL tubes 24 TallPrep™ 13
Standard tube holders
for ambient 5 mL tubes 16 MidiPrep™ 14
temperature work
15 mL tubes 12 TeenPrep™ 14
50 mL tubes 2 BigPrep™ 14
2 mL tubes 24 Metal QuickPrep™ 16
Low & Medium
Throughput 5 mL tubes 18 Metal MidiPrep™ 16
FastPrep® 24s Metal tube holders 15 mL tubes 12 Metal TeenPrep™ 16
- sterilizable
50 mL tubes 2 Metal BigPrep™ 16
2 mL tubes 24 CoolPrep™ 15
Temperature 15 mL tubes 6 CoolTeenPrep™ 15
sensitive
sample 50 mL tubes 2 CoolBigPrep™ 15
96 deep well plates 2 x 96 Dual Plate™ 17
2mL tubes 96 QuickFlex™ 17
4.5 mL tubes 48 TallFlex™ 17
Standard ambient
temperature 15 mL tubes 24 TeenFlex™ 18
High-Throughput
& Volume 50 mL tubes 8 BigFlex™ 18
FastPrep® 96 250 mL bottles 2 LargeFlex™ 18
www.mpbio.comwww.mpbio.com 12
Ambient Tube Holders
Ambient Temperature Sample Grinding with FastPrep-24® and FastPrep-24® 5G Instruments
Ambient temperature tube holders for all your classic and routine grinding.
MP Biomedicals offers the widest selection of tube holders to best meet
your needs in sample lysis and grinding.
Our tube holders allow for sample sizes ranging from 2 mL to 250 mL and are
built for durability in ambient and cryogenic conditions.
QuickPrep™ 1
Cat. N°: 116002512
Capacity: 24 x 2 mL Tubes
QuickPrep™ 3
Cat. N°: 116005512
Capacity: 24 x 2 mL Tubes
HiPrep™
Cat. N°: 116002527
Capacity: 48 x 2 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
TallPrep™
Cat. N°: 116002540
Capacity: 24 x 4.5 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
www.mpbio.comwww.mpbio.com 13
Ambient Tube Holders
Ambient Temperature Sample Grinding with FastPrep-24® and FastPrep-24® 5G Instruments
Ambient temperature tube holders for all your classic and routine grinding.
MidiPrep™
Cat. N°: 116002557
Capacity: 16 x 5 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
TeenPrep™
Cat. N°: 116002526
Capacity: 12 x 15 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
BigPrep™
Cat. N°: 116002525
Capacity: 2 x 50 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
www.mpbio.comwww.mpbio.com 14
Cryogenic Tube Holders
Temperature Sensitive Sample Grinding with FastPrep-24® and FastPrep-24® 5G Instruments
Tube holders for extraction of any sensitive or thermally unstable biological compound.
Cryogenic temperature tube holders for FastPrep-24® Classic and
FastPrep-24® 5G instruments. During mechanical lysis, the temperature
within the tube can increase and cause damage to the molecules in your
sample.
Protects thermosensitive molecules from heat degradation with cooling
chamber.
Prevents the increase of sample temperature during the homogenization
process by maintaining sample temperature at negative temperature.
Ensures a highly effective grinding process of any sample, even the most
elastic, by making them brittle.
CoolPrep™
Cat. N°: 116002528
Capacity: 24 x 2 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
Cool TeenPrep™
Cat. N°: 116002530
Capacity: 6 x 15 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
Cool BigPrep™
Cat. N°: 116002531
Capacity: 2 x 50 mL Tubes
Companion product: Blue Ratchet Nut/CamLock (page 19)
www.mpbio.comwww.mpbio.com 15
Metal Tube Holders
Infectious Sample Grinding with FastPrep-24® Classic and FastPrep-24® 5G Instruments
All metal sample holders are ideally suited for work with highly infectious,
pathogenic, or other biologically hazardous samples.
They withstand temperatures up to 450 °C, allowing for sterilization by
pyrolysis or autoclaving. Pathogens, including bacteria, viruses, fungi,
parasites, viroids, and prions, can be effectively eliminated.
All metal sample holders are also safe to use with most laboratory detergents
and sterilization solutions, ensuring easy care and maintenance.
MetalQuickPrep™
Cat. N°: 116002545
Capacity: 24 x 2 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
MetalMidiPrep™
Cat. N°: 116002544
Capacity: 18 x 5 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
MetalTeenPrep™
Cat. N°: 116002546
Capacity: 12 x 15 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
MetalBigPrep™
Cat. N°: 116002547
Capacity: 2 x 50 mL Tubes
Companion product: Blue Ratchet Nut (page 19)
www.mpbio.com 16
High Capacity Tube Holders
Ambient Temperature Sample Grinding with FastPrep-96® Instruments
FastPrep-96® offers the widest variety of sample holders (2 x 96 deep well
plates, 96 x 2 mL, 48 x 4.5 mL, 24 x 15 mL, 8 x 50 mL and 2 x 250 mL bottles)
and a simple, accurate, closed loop control of lysing power and speed.
All this and more make the FastPrep-96® the perfect solution for all of your
high volume sample preparation needs.
Dual Plate™
Cat. N°: 119696168
Capacity: 2 x 96 Deep Well Plates
QuickFlex™
Cat. N°: 116010570
Capacity: 96 x 2 mL Tubes
TallFlex™
Cat. N°: 116010580
Capacity: 48 x 4.5 mL Tubes
www.mpbio.com 17
High Capacity Tube Holders
Sample Holders for FastPrep-96® Instrument
Ambient temperature tube holders for high-throughput and large volume grinding.
TeenFlex™
Cat. N°: 116010560
Capacity: 20 x 15 mL Tubes
BigFlex™
Cat. N°: 116010550
Capacity: 8 x 50 mL Tubes
LargeFlex™
Cat. N°: 116010590
Capacity: 2 x 250 mL Bottles
ConeFlex™ Adapter
For use of FastPrep-24® tube holders
on FastPrep-96®
Cat. N°: 116010595
For all FastPrep-24® tube holders
www.mpbio.com 18
Locking Systems
Locking Systems for FastPrep-24® Classic & FastPrep-24® 5G Instruments.
MP Biomedicals offers the widest selection of sample holders to best meet
your needs in sample preparation. Our sample holders allow for sample sizes
ranging from 2 mL to 250 mL tube size and are built for durability in ambient
and cryogenic conditions.
Ratchet Nut for FastPrep-24® Classic
Cat. No: 116004510
For FastPrep-24® Classic
Replacement tightening spoked button used to secure
adapters on FastPrep-24® Classic.
Blue Clickmaster, Ratchet Nut
Cat. N°: 116004525
For FastPrep-24® Classic or FastPrep-24® 5G
Accessory for FastPrep-24®Classic and FastPrep-24®
5G instruments. Securing knob used to maintain the
tube holder adapters on the central axis or 3 step
assembly.
Due to its high torque, the Ratchet Nut is particularly
recommended for the heaviest adapters, and especially
for metal tube holders.
Camlock
Cat. N°: 116005588
For FastPrep-24® 5G Instrument
Locking system for the FastPrep-24® 5G instrument.
Modeled after the bike tire quick release, the familiar
mechanism allows:
Rapid sample exchange.
Strong grip.
Uniform clamping force.
Ease-of-use.
www.mpbio.com 19
Frequently Asked Questions
Should I balance the tubes when using a FastPrep® instrument?
No. The FastPrep® is not a centrifuge and you do not need to balance the tubes on adapters.
However, if you do not fill the tube holders completely, avoid having all your tubes in one location.
Place your tubes evenly over all the holes in your adapter. To ensure better stability for the top parts
of the adapters, it is important that the adapter rests on more than two points. We recommend
using these with a minimum of 4 tubes. Use empty tubes if necessary .
There is nothing on the display of the FastPrep-24® Classic or
FastPrep-24® 5G, but it is powered and turned on?
Each device is shipped with the ‘emergency stop’ engaged for safety purposes and will not run until
the emergency stop is disengaged. Disengage the ‘emergency stop’ by turning clockwise until it
pops out. EMERGENCY
STOP
How do I convert from the m/s used to describe FastPrep® motion to
the RPM measurements used by other bead beating systems?
The RPM value used by other bead beating systems refers to rotations per minute. However,
FastPrep® instruments do not use a rotating motion but rather a tridimensional motion. The
FastPrep®’s m/s refers to the distance traveled by the sample or adapter bottom during one second.
To convert from rpm to m/s, compare the number of turns carried out by the axis of the engine
against the meter per second below.
• Motor speed at 4 m/s is equivalent to between 3,400 to 4,100 RPM.
• Motor speed at 6.5 m/s is equivalent to between 3,700 to 4,500 RPM.
Note that this conversion depends on the adapter used and the distance from tubes to the axis of
the FastPrep®.
Can I use and leave my FastPrep® in a cold room at 4 °C for a long
period of time? 4 °C
The operating temperature range for the FastPrep® Instruments is from 2 – 48 °C so storing at
4 °C is not a problem. MP Biomedicals has thoroughly tested using and storing a FastPrep®in a
cold room and have confirmed with other customers. However, it was built to be used at room
temperature so there is a risk of condensed water negatively affecting the motor oil.
Where can I have more technical information or European declaration
of conformity.
Contact us to have more
On our website www.mpbio.com/eu/life-sciences/sample-preparation-0/fastprep-bead-beating-systems informations:
you can download user manual and find all specification. techsup.eur@mpbio.com
For certificates, service manual or any other information about MP Biomedicals FastPrep® please www.mpbio.com
address your request through website to our European technical support team. They will be happy
to help .
www.mpbio.com 20
FastPrep® Lysing Matrix
Experience Unmatched Versatility
For your toughest and most difficult sample types
Plants Animals Soil Yeast and Bacteria Feces
Fungi
2 mL 4.5 mL 15 mL 50 mL 250 mL 96 deep well
Lysing Matrix tubes Lysing Matrix tubes Lysing Matrix tubes Lysing Matrix tubes Lysing Matrix bottles Lysing Matrix plates
FastPrep® Lysing Matrices make difficult-to-lyse samples easy. No matter how tough or resistant
your samples are FastPrep® Lysing Matrix tubes will effectively disrupt cell walls, providing the
highest yields of nucleic acids and proteins in a matter of seconds. All Lysing Matrix tubes fit any
high-speed bead-beating homogenizers. MP Biomedicals offers a wide variety of lysing bead
mixes to fit all sample types and applications.
www.mpbio.comwww.mpbio.comwww.mpbio.com 2121
Standard Lysing Matrix Tubes
Ready-to-use for all your Sample Types
Achieve optimal lysing performance with FastPrep® Lysing Matrix tubes.
FastPrep® Lysing Matrices are critical components of the FastPrep® sample preparation system.
Lysing Matrix tubes come in 16 unique matrix compositions to ensure leak and contamination-free homogenization of any
sample type.
Optimal cell disruption of any sample:The size of the beads and their composition are
optimized according to the sample and the downstream application.
A solution for any application: Unmatched versatility with 16 matrix compositions
available in 2 mL, 4.5 mL, 15 mL, 50 mL, 250 mL bottles or 96-well plates.
High reproducibility: Lysing Matrix tubes contain highly controlled size and quantity of
Lysing Matrix particles and are certified DNase & RNase free.
No cross-contamination: Single-use closed Lysing Matrix tubes.
A gold standard for Life Science applications validated with + 9,000 publications: They
fit any high-speed bead-beating machines.
Because Size Matters
All Lysing Matrix tubes offered come in every size, from 2 mL to 250 mL as well as 96 deep well plates.
Up to Up to Up to Up to Up to
150 mg 500 mg 1 g 3 g 50 g
96 deep well plates 2 mL tube 4.5 mL tube 15 mL tube 50 mL tube 250 mL bottle
Description Pack Size
2 mL 50, 100 and 500 x 2 mL tubes
N
R a
s
4.5 mL 25, 50 and 100 x 4.5 mL tubes /
e e
s F
a R
N E
15 mL 25 and 50 x 15 mL tubes D E
50 mL 10, 50 and 100 x 50 mL tubes
C D
250 mL 10 x 250 mL bottles E
R E
I
T I F
96 deep well plates 1 and 10 x 96 deep well plates
www.mpbio.com 22
Lysing Matrix Tubes Selection Guide
Find the Lysing Matrix Tailored to your Application
Sample Type Lysing Matrix
Animal & Human Tissues A B C D E F G H I J K M S SS Y Z
Lung, Breast, Kidney, Heart,
Soft Tissues Intestine, Muscle,
Spleen, Liver, Brain
Skin
Nail
Tail & Ear
Vascular Tissue
Unique Hair
Samples
Bone
Tumor
Mammalian Cell
Infected Tissue (isolation of
viruses or virus)
Microorganisms A B C D E F G H I J K M S SS Y Z
Bacteria (gram + and -)
Yeast & Mold
Bacterial & Fungal Spore
Algae
Virus
Environmental Samples A B C D E F G H I J K M S SS Y Z
Soil, Marine Sediment,
Rhizosphere, Manure,
Compost, Sludge, Feces,
Wastewater
Plant Tissues A B C D E F G H I J K M S SS Y Z
Leaf
Seed
Root
Needle
Wood
Stem & Flower
Insects & Worms A B C D E F G H I J K M S SS Y Z
Tick & Fly
Nematode
Bee & Mosquito
www.mpbio.com 23
Lysing Matrix Tubes
Experience Unmatched Versatility
Achieve optimal lysing performance with FastPrep® Lysing Matrix tubes.
Lysing Matrix A Lysing Matrix B Lysing Matrix C
Composition: Garnet matrix and one Composition: 0.1 mm silica spheres Composition: 1 mm silica spheres
6.35 mm ceramic sphere
All sample types Animal Fungi Bacteria Spores Yeast Fungi Algae
Bacteria Yeast Plants
Lysing Matrix D Lysing Matrix E Lysing Matrix F
Composition: 1.4 mm ceramic spheres Composition: 1.4 mm ceramic spheres, Composition: 1.6 mm aluminum oxide
0.1 mm silica spheres and 4 mm glass bead particles and 1.6 mm silicon carbide particles
Animal tissues Plants tissues Soil Fecal Sludge Pollen Animal tissues Plants tissues
Cultured cells Wastewater Environmental water Bacteria Mold & fungi
Lysing Matrix G Lysing Matrix H Lysing Matrix I
Composition: 1.6 mm silicon carbide Composition: 2 mm glass beads and 2 mm Composition: 2 mm yellow zirconia beads
particles and 2 mm glass beads yellow zirconium oxide beads and one 4 mm ceramic sphere
Animal Plants Ancient & dried samples
Animal tissues Plants tissues Animal Plants Coral emulsions
Very dense soil and clay Bacterial aggregates
Yeast Fungi Molds & fungi Fixed samples
Whole insects, tickes & fleas Wood & seeds
www.mpbio.com 24
Lysing Matrix Tubes
Experience Unmatched Versatility
Achieve optimal lysing performance with FastP
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Date d'upload du document :
mardi 30 janvier 2024
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